Pandamooz, Sareh and Salehi, Mohammad Saied and Jurek, Benjamin and Meinung, Carl-Philipp and Azarpira, Negar and Dianatpour, Mehdi and Neumann, Inga D. (2023) Oxytocin Receptor Expression in Hair Follicle Stem Cells: A Promising Model for Biological and Therapeutic Discovery in Neuropsychiatric Disorders. STEM CELL REVIEWS AND REPORTS, 19 (7). pp. 2510-2524. ISSN 2629-3269, 2629-3277
Full text not available from this repository. (Request a copy)Abstract
The intricate nature of the human brain and the limitations of existing model systems to study molecular and cellular causes of neuropsychiatric disorders represent a major challenge for basic research. The promising progress in patient-derived stem cell technology and in our knowledge on the role of the brain oxytocin (OXT) system in health and disease offer new possibilities in that direction. In this study, the rat hair follicle stem cells (HFSCs) were isolated and expanded in vitro. The expression of oxytocin receptors (OXTR) was evaluated in these cells. The cellular viability was assessed 12 h post stimulation with OXT. The activation of OXTR-coupled intracellular signaling cascades, following OXT treatment was determined. Also, the influence of OXT on neurite outgrowth and cytoskeletal rearrangement were defined. The assessment of OXTR protein expression revealed this receptor is expressed abundantly in HFSCs. As evidenced by the cell viability assay, no adverse or cytotoxic effects were detected following 12 h treatment with different concentrations of OXT. Moreover, OXTR stimulation by OXT resulted in ERK1/2, CREB, and eEF2 activation, neurite length alterations, and cytoskeletal rearrangements that reveal the functionality of this receptor in HFSCs. Here, we introduced the rat HFSCs as an easy-to-obtain stem cell model that express functional OXTR. This cell-based model can contribute to our understanding of the progression and treatment of neuropsychiatric disorders with oxytocinergic system deficiency.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEURONAL CELLS; TRANSCRIPTION; CYTOSKELETON; TRAFFICKING; ORGANOIDS; LENGTH; NCSC; Hair follicle Stem Cell; Oxytocin Receptor; Oxytocin; Psychopathology; Model System |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Mar 2024 09:27 |
| Last Modified: | 13 Mar 2024 09:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/59861 |
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