Asami, Maki and Lam, Brian Y. H. and Hoffmann, Martin and Suzuki, Toru and Lu, Xin and Yoshida, Naoko and Ma, Marcella K. and Rainbow, Kara and Guz, Miodrag and VerMilyea, Matthew D. and Yeo, Giles S. H. and Klein, Christoph A. and Perry, Anthony C. F. (2023) A program of successive gene expression in mouse one-cell embryos. CELL REPORTS, 42 (2): 112023. ISSN 2211-1247,
Full text not available from this repository. (Request a copy)Abstract
At the moment of union in fertilization, sperm and oocyte are transcriptionally silent. The ensuing onset of embryonic transcription (embryonic genome activation [EGA]) is critical for development, yet its timing and profile remain elusive in any vertebrate species. We here dissect transcription during EGA by high -res-olution single-cell RNA sequencing of precisely synchronized mouse one-cell embryos. This reveals a pro-gram of embryonic gene expression (immediate EGA [iEGA]) initiating within 4 h of fertilization. Expression during iEGA produces canonically spliced transcripts, occurs substantially from the maternal genome, and is mostly downregulated at the two-cell stage. Transcribed genes predict regulation by transcription factors (TFs) associated with cancer, including c-Myc. Blocking c-Myc or other predicted regulatory TF activities dis-rupts iEGA and induces acute developmental arrest. These findings illuminate intracellular mechanisms that regulate the onset of mammalian development and hold promise for the study of cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | C-MYC; TRANSCRIPTIONAL ACTIVITY; TERMINAL DOMAIN; ALPHA-TUBULIN; CHROMATIN; GENOME; ACTIVATION; PHOSPHORYLATION; GROWTH; FERTILIZATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für experimentelle Medizin und Therapieverfahren |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 10:05 |
| Last Modified: | 30 Jan 2024 10:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60016 |
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