Natali, Lautaro and Pizarro, Gabriel Luna and Moyano, Sofia and de la Cruz-Thea, Benjamin and Musso, Juliana and Ropolo, Andrea S. and Eichner, Norbert and Meister, Gunter and Musri, Melina M. and Feliziani, Constanza and Touz, Maria C. (2023) The Exosome-like Vesicles of Giardia Assemblages A, B, and E Are Involved in the Delivering of Distinct Small RNA from Parasite to Parasite. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (11): 9559. ISSN 1661-6596, 1422-0067
Full text not available from this repository. (Request a copy)Abstract
The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. The Giardia assemblages are separated by large genetic distances, which might account for their relevant biological and pathogenic differences. In this work, we analyzed the RNAs cargo released into exosomal-like vesicles (ElVs) by the assemblages A and B, which differentially infect humans, and the assemblage E, which infects hoofed animals. The RNA sequencing analysis revealed that the ElVs of each assemblage contained distinct small RNA (sRNA) biotypes, suggesting a preference for specific packaging in each assemblage. These sRNAs were classified into three categories, ribosomal-small RNAs (rsRNAs), messenger-small RNAs (msRNAs), and transfer-small RNAs (tsRNAs), which may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis. Uptake experiments showed, for the first time, that ElVs were successfully internalized by the parasite trophozoites. Furthermore, we observed that the sRNAs contained inside these ElVs were first located below the plasma membrane but then distributed along the cytoplasm. Overall, the study provides new insights into the molecular mechanisms underlying the host-specificity and pathogenesis of G. lamblia and highlights the potential role of sRNAs in parasite communication and regulation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACELLULAR VESICLES; IN-VITRO; BIOGENESIS; INTESTINALIS; INFECTION; REVEALS; LAMBLIA; PROTEIN; ROLES; CARGO; Giardia lamblia; parasite to parasite communication; exosomal-like vesicles (ElVs); small RNAs (sRNA); msRNA; tsRNA; rsRNA; trophozoites; host-specificity; pathogenesis |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Mar 2024 11:54 |
| Last Modified: | 16 Mar 2024 11:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60155 |
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