Keckstein, Simon and Tilgener, Constantin and Jeschke, Udo and Hofmann, Simone and Vilsmaier, Theresa and Keilmann, Lucia and Heidegger, Helene and Kaltofen, Till and Batz, Falk and Mahner, Sven and Schroeder, Lennard (2024) Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells. ARCHIVES OF GYNECOLOGY AND OBSTETRICS, 309. pp. 1509-1514. ISSN 0932-0067, 1432-0711
Full text not available from this repository. (Request a copy)Abstract
Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ER beta). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 mu g/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ER beta was quantified at gene level by realtime polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 mu g/ml. The WB demonstrated a significant quantitative decrease of ER beta at protein level with MTE concentrations of 10 mu g/ml. In contrast, the PCR did not result in significant downregulation of ER beta. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ER beta at protein level.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHYTOESTROGENS; Matcha tea extract; MTE; MCF-7; ER beta; WST-1; PCR; Western blot |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2024 05:02 |
| Last Modified: | 04 Mar 2025 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60202 |
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