Huang, Youmei and Liu, Liping and Chai, Mengnan and Su, Han and Ma, Suzhuo and Liu, Kaichuang and Tian, Yaru and Cao, Zhuangyuan and Xi, Xinpeng and Zhu, Wenhui and Qi, Jingang and Palanivelu, Ravishankar and Qin, Yuan and Cai, Hanyang (2023) Epigenetic regulation of female germline development through ERECTA signaling pathway. NEW PHYTOLOGIST, 240 (3). pp. 1015-1033. ISSN 0028-646X, 1469-8137
Full text not available from this repository. (Request a copy)Abstract
Germline development is a key step in sexual reproduction. Sexual plant reproduction begins with the formation of haploid spores by meiosis of megaspore mother cells (MMCs). Although many evidences, directly or indirectly, show that epigenetics plays an important role in MMC specification, how it controls the commitment of the MMC to downstream stages of germline development is still unclear. Electrophoretic mobility shift assay (EMSA), western blot, immunofluorescence, and chromatin immunoprecipitation coupled with quantitative PCR analyses were performed. Genetic interactions between BZR1 transcription factor family and the SWR1-SDG2-ER pathway in the control of female germline development were further studied. The present findings showed in Arabidopsis that two epigenetic factors, the chromatin remodeling complex SWI2/SNF2-RELATED 1 (SWR1) and a writer for H3K4me3 histone modification SET DOMAIN GROUP 2 (SDG2), genetically interact with the ERECTA (ER) receptor kinase signaling pathway and regulate female germline development by restricting the MMC cell fate to a single cell in the ovule primordium and ensure that only that single cell undergoes meiosis and subsequent megaspore degeneration. We also showed that SWR1-SDG2-ER signaling module regulates female germline development by promoting the protein accumulation of BZR1 transcription factor family on the promoters of primary miRNA processing factors, HYPONASTIC LEAVES 1 (HYL1), DICER-LIKE 1 (DCL1), and SERRATE (SE) to activate their expression. Our study elucidated a Gene Regulation Network that provides new insights for understanding how epigenetic factors and receptor kinase signaling pathways function in concert to control female germline development in Arabidopsis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE-EXPRESSION; PLANT-GROWTH; CELL FATE; ARABIDOPSIS; SPECIFICATION; METHYLATION; ACTIVATION; TRANSITION; MEIOSIS; NETWORK; Arabidopsis; BZR1; ERECTA; megaspore mother cells; SDG2; SWR1 |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Mar 2024 08:45 |
| Last Modified: | 23 Mar 2024 08:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60300 |
Actions (login required)
 |
View Item |
