Balk, Stefan and Koenig, Burkhard (2015) Thermally induced molecular imprinting of luminescent vesicles. JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY, 81 (1-2). pp. 135-139. ISSN 1388-3127, 1573-1111
Full text not available from this repository. (Request a copy)Abstract
Thermal imprinting of the lipid-water interface of phospholipid vesicles is achieved by reversible non-covalent assembly of membrane embedded amphiphilic metal complexes. The complexes have affinity to phosphate and imidazole groups and are preorganized by a phosphorylated hexapeptide template above the phase transition temperature. The template induced patterning is transferred into the gel phase of the membrane by cooling below the transition temperature. This limits the lateral diffusion and stabilizes the metal complex receptor organization, as confirmed by FRET measurements with dye-labeled receptors. After template removal an enhanced rebinding affinity of one order of magnitude for the target peptide was observed for the imprinted membranes compared to identical non-imprinted interfaces.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENZYME-INHIBITORS; BINDING-SITES; PHOSPHOLIPID-BILAYERS; POLYMERS; MEMBRANES; RECOGNITION; ADSORPTION; ANTIBODIES; RECEPTORS; PROTEINS; Non-covalent imprinting; Template induced patterning; Functionalized vesicles; Specific recognition sites; FRET; Emission titrations |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jul 2019 07:29 |
| Last Modified: | 25 Jul 2019 07:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6043 |
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