Rux, Cassandra and Wittmer, Annette and Stork, Anja and Vach, Kirstin and Hellwig, Elmar and Cieplik, Fabian and Al-Ahmad, Ali (2023) Optimizing the use of low-frequency ultrasound for bacterial detachment of in vivo biofilms in dental research-a methodological study. CLINICAL ORAL INVESTIGATIONS, 28 (1): 19. ISSN 1432-6981, 1436-3771
Full text not available from this repository. (Request a copy)Abstract
ObjectivesLow-frequency, low-intensity ultrasound is commonly utilized in various dental research fields to remove biofilms from surfaces, but no clear recommendation exists in dental studies so far. Therefore, this study aims to optimize the sonication procedure for the dental field to efficiently detach bacteria while preserving viability.Materials and methodsInitial biofilm was formed in vivo on bovine enamel slabs (n = 6) which were worn by four healthy participants for 4 h and 24 h. The enamel slabs covered with biofilm were then ultrasonicated ex vivo for various time periods (0, 1, 2, 4, 6 min). Colony-forming units were determined for quantification, and bacteria were identified using MALDI-TOF. Scanning electron microscopic images were taken to also examine the efficiency of ultrasonications for different time periods.ResultsUltrasonication for 1 min resulted in the highest bacterial counts, with at least 4.5-fold number compared to the non-sonicated control (p < 0.05). Most bacteria were detached within the first 2 min of sonication, but there were still bacteria detached afterwards, although significantly fewer (p < 0.0001). The highest bacterial diversity was observed after 1 and 2 min of sonication (p < 0.03). Longer sonication periods negatively affected bacterial counts of anaerobes, Gram-negative bacteria, and bacilli. Scanning electron microscopic images demonstrated the ability of ultrasound to desorb microorganisms, as well as revealing cell damage and remaining bacteria.ConclusionsWith the use of low-frequency, low-intensity ultrasound, significantly higher bacterial counts and diversity can be reached. A shorter sonication time of 1 min shows the best results overall.Clinical relevanceThis standardization is recommended to study initial oral biofilms aged up to 24 h to maximize the outcome of experiments and lead to better comparability of studies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SONICATION; DIAGNOSIS; ADHESION; COLONIZATION; PLAQUE; VITRO; QUANTIFICATION; INFECTION; ADHERENCE; CULTURE; Low-frequency ultrasound; Biofilm removal; Desorption; Sonication; Initial biofilm; Bovine enamel slabs |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Mar 2024 12:26 |
| Last Modified: | 23 Mar 2024 12:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60453 |
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