Farka, Zdenek and Brandmeier, Julian C. and Mickert, Matthias J. and Pastucha, Matej and Lacina, Karel and Skladal, Petr and Soukka, Tero and Gorris, Hans H. (2024) Nanoparticle-Based Bioaffinity Assays: From the Research Laboratory to the Market. ADVANCED MATERIALS, 36 (3). p. 2307653. ISSN 0935-9648, 1521-4095
Full text not available from this repository. (Request a copy)Abstract
Advances in the development of new biorecognition elements, nanoparticle-based labels as well as instrumentation have inspired the design of new bioaffinity assays. This review critically discusses the potential of nanoparticles to replace current enzymatic or molecular labels in immunoassays and other bioaffinity assays. Successful implementations of nanoparticles in commercial assays and the need for rapid tests incorporating nanoparticles in different roles such as capture support, signal generation elements, and signal amplification systems are highlighted. The limited number of nanoparticles applied in current commercial assays can be explained by challenges associated with the analysis of real samples (e.g., blood, urine, or nasal swabs) that are difficult to resolve, particularly if the same performance can be achieved more easily by conventional labels. Lateral flow assays that are based on the visual detection of the red-colored line formed by colloidal gold are a notable exception, exemplified by SARS-CoV-2 rapid antigen tests that have moved from initial laboratory testing to widespread market adaption in less than two years. This review critically discusses advances in nanoparticle-based labels (yellow) combined with biorecognition elements (green) and suitable readout schemes for the design of new bioaffinity assays. The route of nanoparticle-based assays from the bench to real sample analysis and commercialization follows a rugged path winding between many challenges and benefits.image
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LATERAL FLOW IMMUNOASSAY; PHOTON-UPCONVERTING NANOPARTICLES; MOLECULARLY IMPRINTED POLYMERS; UP-CONVERSION NANOPARTICLES; LINKED-IMMUNOSORBENT-ASSAY; TIME-RESOLVED FLUORESCENCE; QUANTUM DOTS; NUCLEIC-ACID; SEMICONDUCTOR NANOCRYSTALS; HOMOGENEOUS IMMUNOASSAY; biosensor; Covid-19; digital assay; immunoassay; nanoparticle; point-of-care testing |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Apr 2024 05:55 |
| Last Modified: | 04 Mar 2025 09:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60455 |
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