Lan, Zijun and Song, Zihan and Wang, Zhijuan and Li, Ling and Liu, Yiqun and Zhi, Shuaihua and Wang, Ruihan and Wang, Jizong and Li, Qiyun and Bleckmann, Andrea and Zhang, Li and Dresselhaus, Thomas and Dong, Juan and Gu, Hongya and Zhong, Sheng and Qu, Li-Jia (2023) Antagonistic RALF peptides control an intergeneric hybridization barrier on Brassicaceae stigmas. CELL, 186 (22). 4773-+. ISSN 0092-8674, 1097-4172
Full text not available from this repository. (Request a copy)Abstract
Pollen-pistil interactions establish interspecific/intergeneric pre-zygotic hybridization barriers in plants. The rejection of undesired pollen at the stigma is crucial to avoid outcrossing but can be overcome with the sup-port of mentor pollen. The mechanisms underlying this hybridization barrier are largely unknown. Here, in Arabidopsis, we demonstrate that receptor-like kinases FERONIA/CURVY1/ANJEA/HERCULES RECEPTOR KINASE 1 and cell wall proteins LRX3/4/5 interact on papilla cell surfaces with autocrine stigmatic RALF1/22/ 23/33 peptide ligands (sRALFs) to establish a lock that blocks the penetration of undesired pollen tubes. Compatible pollen-derived RALF10/11/12/13/25/26/30 peptides (pRALFs) act as a key, outcompeting sRALFs and enabling pollen tube penetration. By treating Arabidopsis stigmas with synthetic pRALFs, we unlock the barrier, facilitating pollen tube penetration from distantly related Brassicaceae species and result-ing in interspecific/intergeneric hybrid embryo formation. Therefore, we uncover a "lock-and-key"system governing the hybridization breadth of interspecific/intergeneric crosses in Brassicaceae. Manipulating this system holds promise for facilitating broad hybridization in crops.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLLEN-TUBE GROWTH; SELF-INCOMPATIBILITY; REPRODUCTIVE ISOLATION; PISTIL INTERACTIONS; LRX PROTEINS; ARABIDOPSIS; INTEGRITY; FERONIA; INSIGHTS; ATRBOHD; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 10:20 |
| Last Modified: | 30 Jan 2024 10:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60470 |
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