Wolf, Elena E. E. and Steglich, Anne and Kessel, Friederike and Kroeger, Hannah and Sradnick, Jan and Reichelt-Wurm, Simone and Eidenschink, Kathrin and Banas, Miriam C. C. and Wolf, Eckhard and Wanke, Ruediger and Gembardt, Florian and Todorov, Vladimir T. T. (2023) PLVAP as an Early Marker of Glomerular Endothelial Damage in Mice with Diabetic Kidney Disease. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2): 1094. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Plasmalemma vesicle-associated protein (PLVAP) is the main component of endothelial diaphragms in fenestrae, caveolae, and transendothelial channels. PLVAP is expressed in the adult kidney glomerulus upon injury. Glomerular endothelial injury is associated with progressive loss of kidney function in diabetic kidney disease (DKD). This study aimed to investigate whether PLVAP could serve as a marker for glomerular endothelial damage in DKD. Glomerular PLVAP expression was analyzed in different mouse models of DKD and their respective healthy control animals using automatic digital quantification of histological whole kidney sections. Transgenic mice expressing a dominant-negative GIP receptor (GIPR(dn)) in pancreatic beta-cells as a model for diabetes mellitus (DM) type 1 and black and tan brachyuric (BTBR) ob/ob mice, as a model for DM type 2, were used. Distinct PLVAP induction was observed in all diabetic models studied. Traces of glomerular PLVAP expression could be identified in the healthy control kidneys using automated quantification. Stainings for other endothelial injury markers such as CD31 or the erythroblast transformation-specific related gene (ERG) displayed no differences between diabetic and healthy groups at the time points when PLVAP was induced. The same was also true for the mesangial cells marker alpha 8Integrin, while the podocyte marker nephrin appeared to be diminished only in BTBR ob/ob mice. Glomerular hypertrophy, which is one of the initial morphological signs of diabetic kidney damage, was observed in both diabetic models. These findings suggest that PLVAP is an early marker of glomerular endothelial injury in diabetes-induced kidney damage in mice.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DIAPHRAGMS; PODOCYTE; SURFACE; PV-1; MECHANISMS; COMPONENT; PATHWAYS; INJURY; MOUSE; IMAGE; diabetic kidney disease; endothelial damage; PLVAP; diabetic models; glomerular hypertrophy |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Mar 2024 13:14 |
| Last Modified: | 23 Mar 2024 13:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60486 |
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