Blanco-Carmona, Enrique and Narayanan, Ashwin and Hernandez, Inmaculada and Nieto, Juan C. and Elosua-Bayes, Marc and Sun, Xueyuan and Schmidt, Claudia and Pamir, Necmettin and Ozduman, Koray and Herold-Mende, Christel and Pagani, Francesca and Cominelli, Manuela and Taranda, Julian and Wick, Wolfgang and von Deimling, Andreas and Poliani, Pietro Luigi and Rehli, Michael and Schlesner, Matthias and Heyn, Holger and Turcan, Sevin (2023) Tumor heterogeneity and tumor-microglia interactions in primary and recurrent IDH1-mutant gliomas. CELL REPORTS MEDICINE, 4 (11): 101249. ISSN 2666-3791,
Full text not available from this repository. (Request a copy)Abstract
The isocitrate dehydrogenase (IDH) gene is recurrently mutated in adult diffuse gliomas. IDH-mutant gliomas are categorized into oligodendrogliomas and astrocytomas, each with unique pathological features. Here, we use single-nucleus RNA and ATAC sequencing to compare the molecular heterogeneity of these glioma subtypes. In addition to astrocyte-like, oligodendrocyte progenitor-like, and cycling tumor subpopulations, a tumor population enriched for ribosomal genes and translation elongation factors is primarily present in oligodendrogliomas. Longitudinal analysis of astrocytomas indicates that the proportion of tumor subpopulations remains stable in recurrent tumors. Analysis of tumor-associated microglia/macrophages (TAMs) reveals significant differences between oligodendrogliomas, with astrocytomas harboring inflammatory TAMs expressing phosphorylated STAT1, as confirmed by immunohistochemistry. Furthermore, inferred receptor-ligand interactions between tumor subpopulations and TAMs may contribute to TAM state diversity. Overall, our study sheds light on distinct tumor populations, TAM heterogeneity, TAM-tumor interactions in IDH-mutant glioma subtypes, and the relative stability of tumor subpopulations in recurrent astrocytomas.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STIMULATING FACTOR-I; SINGLE-CELL; MUTATIONS; LANDSCAPE; HIERARCHY; GROWTH; IDH1; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 10:10 |
| Last Modified: | 30 Jan 2024 10:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60495 |
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