Sahana, Jayashree and Cortes-Sanchez, Jose Luis and Sandt, Viviann and Melnik, Daniela and Corydon, Thomas J. and Schulz, Herbert and Cai, Zexi and Evert, Katja and Grimm, Daniela and Wehland, Markus (2023) Long-Term Simulation of Microgravity Induces Changes in Gene Expression in Breast Cancer Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (2): 1181. ISSN 1661-6596, 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Microgravity changes the gene expression pattern in various cell types. This study focuses on the breast cancer cell lines MCF-7 (less invasive) and MDA-MB-231 (triple-negative, highly invasive). The cells were cultured for 14 days under simulated microgravity (s-mu g) conditions using a random positioning machine (RPM). We investigated cytoskeletal and extracellular matrix (ECM) factors as well as focal adhesion (FA) and the transmembrane proteins involved in different cellular signaling pathways (MAPK, PAM and VEGF). The mRNA expressions of 24 genes of interest (TUBB, ACTB, COL1A1, COL4A5, LAMA3, ITGB1, CD44, VEGF, FLK1, EGFR, SRC, FAK1, RAF1, AKT1, ERK1, MAPK14, MAP2K1, MTOR, RICTOR, VCL, PXN, CDKN1, CTNNA1 and CTNNB1) were determined by quantitative real-time PCR (qPCR) and studied using STRING interaction analysis. Histochemical staining was carried out to investigate the morphology of the adherent cells (ADs) and the multicellular spheroids (MCSs) after RPM exposure. To better understand this experimental model in the context of breast cancer patients, a weighted gene co-expression network analysis (WGCNA) was conducted to obtain the expression profiles of 35 breast cell lines from the HMS LINCS Database. The qPCR-verified genes were searched in the mammalian phenotype database and the human genome-wide association studies (GWAS) Catalog. The results demonstrated the positive association between the real metastatic microtumor environment and MCSs with respect to the extracellular matrix, cytoskeleton, morphology, different cellular signaling pathway key proteins and several other components. In summary, the microgravity-engineered three-dimensional MCS model can be utilized to study breast cancer cell behavior and to assess the therapeutic efficacies of drugs against breast cancer in the future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENOME-WIDE ASSOCIATION; HUMAN ENDOTHELIAL-CELLS; 3-DIMENSIONAL GROWTH; BETA-CATENIN; SUSCEPTIBILITY LOCI; PHOSPHORYLATION; INTEGRINS; MIGRATION; SYSTEM; breast cancer; spheroids; cytoskeleton; extracellular matrix; focal adhesion; microgravity; PAM signaling |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2024 06:08 |
| Last Modified: | 09 Apr 2024 06:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60514 |
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