Ma, Lijun and Shelness, Gregory S. and Snipes, James A. and Murea, Mariana and Antinozzi, Peter A. and Cheng, Dongmei and Saleem, Moin A. and Satchell, Simon C. and Banas, Bernhard and Mathieson, Peter W. and Kretzler, Matthias and Hemal, Ashok K. and Rudel, Lawrence L. and Petrovic, Snezana and Weckerle, Allison and Pollak, Martin R. and Ross, Michael D. and Parks, John S. and Freedman, Barry I. (2015) Localization of APOL1 Protein and mRNA in the Human Kidney: Nondiseased Tissue, Primary Cells, and Immortalized Cell Lines. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 26 (2). pp. 339-348. ISSN 1046-6673, 1533-3450
Full text not available from this repository. (Request a copy)Abstract
Although APOL1 gene variants are associated with nephropathy in African Americans, little is known about APOL1 protein synthesis, uptake, and localization in kidney cells. To address these questions, we examined APOL1 protein and nnRNA localization in human kidney and human kidney-derived cell lines. Indirect immunofluorescence microscopy performed on nondiseased nephrectonny cryosections from persons with normal kidney function revealed that APOL1 protein was markedly enriched in podocytes (colocalized with synaptopodin and Wilms' tumor suppressor) and present in lower abundance in renal tubule cells. Fluorescence in situ hybridization detected APOL1 mRNA in glomeruli (podocytes and endothelial cells) and tubules, consistent with endogenous synthesis in these cell types. When these analyses were extended to renal-derived cell lines, quantitative RT-PCR did not detect APOL1 mRNA in human mesangial cells; however, abundant levels of APOL1 mRNA were observed in proximal tubule cells and glomerular endothelial cells, with lower expression in podocytes. Western blot analysis revealed corresponding levels of APOL1 protein in these cell lines. To explain the apparent discrepancy between the marked abundance of APOL1 protein in kidney podocytes observed in cryosections versus the lesser abundance in podocyte cell lines, we explored APOL1 cellular uptake. APOL1 protein was taken up readily by human podocytes in vitro but was not taken up efficiently by mesangial cells, glomerular endothelial cells, or proximal tubule cells. We hypothesize that the higher levels of APOL1 protein in human cryosectioned podocytes may reflect both endogenous protein synthesis and APOL1 uptake from the circulation or glomerular filtrate.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | APOLIPOPROTEIN-L GENE; AFRICAN-AMERICANS; ALLOGRAFT SURVIVAL; ENDOTHELIAL-CELLS; MESANGIAL CELLS; EXPRESSION; DISEASE; IDENTIFICATION; NEPHROPATHY; VARIANTS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jul 2019 08:13 |
| Last Modified: | 25 Jul 2019 08:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6055 |
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