Hoelz, Hannes and Heetmeyer, Jeannine and Tsakmaklis, Anastasia and Hiergeist, Andreas and Siebert, Kolja and De Zen, Federica and Haecker, Deborah and Metwaly, Amira and Neuhaus, Klaus and Gessner, Andre and Vehreschild, Maria J. G. T. and Haller, Dirk and Schwerd, Tobias (2023) Is Autologous Fecal Microbiota Transfer after Exclusive Enteral Nutrition in Pediatric Crohn's Disease Patients Rational and Feasible? Data from a Feasibility Test. NUTRIENTS, 15 (7): 1742. ISSN , 2072-6643
Full text not available from this repository. (Request a copy)Abstract
Background: Exclusive enteral nutrition (EEN) is a highly effective therapy for remission induction in pediatric Crohn's disease (CD), but relapse rates after return to a regular diet are high. Autologous fecal microbiota transfer (FMT) using stool collected during EEN-induced clinical remission might represent a novel approach to maintaining the benefits of EEN. Methods: Pediatric CD patients provided fecal material at home, which was shipped at 4 degrees C to an FMT laboratory for FMT capsule generation and extensive pathogen safety screening. The microbial community composition of samples taken before and after shipment and after encapsulation was characterized using 16S rRNA amplicon sequencing. Results: Seven pediatric patients provided fecal material for nine test runs after at least three weeks of nutritional therapy. FMT capsules were successfully generated in 6/8 deliveries, but stool weight and consistency varied widely. Transport and processing of fecal material into FMT capsules did not fundamentally change microbial composition, but microbial richness was <30 genera in 3/9 samples. Stool safety screening was positive for potential pathogens or drug resistance genes in 8/9 test runs. Conclusions: A high pathogen burden, low-diversity microbiota, and practical deficiencies of EEN-conditioned fecal material might render autologous capsule-FMT an unsuitable approach as maintenance therapy for pediatric CD patients.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; ACTIVE ULCERATIVE-COLITIS; INTESTINAL MICROBIOTA; TROPHERYMA-WHIPPLEI; SUSTAINED REMISSION; TRANSPLANTATION; THERAPY; INDUCTION; CHILDREN; METAANALYSIS; pediatric IBD; Crohn's disease; fecal microbiota transfer; autologous FMT; exclusive enteral nutrition |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2024 08:12 |
| Last Modified: | 26 Mar 2024 08:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60562 |
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