Karimi-Haghighi, Saeideh and Pandamooz, Sareh and Jurek, Benjamin and Fattahi, Sadegh and Safari, Anahid and Azarpira, Negar and Dianatpour, Mehdi and Hooshmandi, Etrat and Bayat, Mahnaz and Owjfard, Maryam and Zafarmand, Seyedeh Shaghayegh and Mostaghel, Mandana and Mousavi, Seyedeh Maryam and Jashire Nezhad, Nahid and Eraghi, Vida and Fadakar, Nima and Rahimi Jaberi, Abbas and Garcia-Esperon, Carlos and Spratt, Neil and Levi, Christopher and Salehi, Mohammad Saied and Borhani-Haghighi, Afshin (2023) From Hair to the Brain: The Short-Term Therapeutic Potential of Human Hair Follicle-Derived Stem Cells and Their Conditioned Medium in a Rat Model of Stroke. MOLECULAR NEUROBIOLOGY, 60 (5). pp. 2587-2601. ISSN 0893-7648, 1559-1182
Full text not available from this repository. (Request a copy)Abstract
The short-term therapeutic impacts of stem cells and their derivatives were frequently reported in preclinical investigations of ischemic stroke (IS); however, several drawbacks including accessibility, abundancy, and ethical concerns limited their clinical application. We describe here for the first time the therapeutic potential of human hair follicle-derived stem cells (hHFSCs) and their conditioned medium (CM) in a rat model of IS. Furthermore, we hypothesized that a combination of cell therapy with repeated CM administration might enhance the restorative efficiency of this approach compared to each treatment alone. Middle cerebral artery occlusion was performed for 30 min to induce IS. Immediately after reperfusion, hHFSCs were transplanted through the intra-arterial route and/or hHFSC-CM administered intranasally. The neurological outcomes, short-term spatial working memory, and infarct size were evaluated. Furthermore, relative expression of seven target genes in three categories of neuronal markers, synaptic markers, and angiogenic markers was assessed. The hHFSCs and hHFSC-CM treatments improved neurological impairments and reduced infarct size in the IS rats. Moreover, molecular data elucidated that IS was accompanied by attenuation in the expression of neuronal and synaptic markers in the evaluated brain regions and the interventions rescued these expression changes. Although there was no considerable difference between hHFSCs and hHFSC-CM treatments in the improvement of neurological function and decrement of infarct size, combination therapy was more effective to reduce infarction and elevation of target gene expression especially in the hippocampus. These findings highlight the curative potential of hHFSCs and their CM in a rat model of IS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STROMAL CELLS; FUNCTIONAL RECOVERY; CEREBRAL-ISCHEMIA; TRANSPLANTATION; THERAPIES; DISEASE; Cerebral ischemia; Conditioned medium; Cell therapy; Epidermal neural crest stem cell; Secretome |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2024 09:53 |
| Last Modified: | 26 Mar 2024 09:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60568 |
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