Neumann, Bernd and Stuerhof, Claudia and Rath, Anca and Kieninger, Baerbel and Eger, Elias and Mueller, Justus U. and von Poblocki, Alexander and Gerlitz, Nadja and Wollschlaeger, Paul and Schneider-Brachert, Wulf and Schaufler, Katharina and Klaper, Kathleen and Steinmann, Joerg (2023) Detection and characterization of putative hypervirulent <i>Klebsiella pneumoniae</i> isolates in microbiological diagnostics. SCIENTIFIC REPORTS, 13 (1): 19025. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Hypervirulent Klebsiella pneumoniae strains (hvKp) can cause invasive community-acquired infections in healthy patients of all ages. In this study, the prevalence of putative hvKp in a German tertiary center was investigated and hvKp were characterized by phenotypic and molecular assays. All K. pneumoniae isolates in routine microbiological diagnostics from a single center were screened by string-testing over a period of 6 months. String-test positive (>= 0.5 mm) isolates were re-evaluated on different media and under various conditions (aerobe, anaerobe). For string-test positive isolates, genes (magA, iutA, rmpA and rmpA2) associated with hypermucoviscosity and hypervirulence were amplified by multiplex PCR. PCR-positive isolates were subjected to whole-genome sequencing and sedimentation and biofilm formation assays. From 1310 screened K. pneumoniae isolates in clinical routine 100 isolates (7.6%) were string test positive. From these, 9% (n=9) were defined as putative hvKp (string-test+/PCR+). Highest rate of string-test-positive isolates was observed on MacConkey agar under aerobic conditions. Amongst these nine putative hvKp isolates, the international lineage ST23 carrying hvKp-plasmid pKpVP-1 was the most common, but also a rare ST86 with pKpVP-2 was identified. All nine isolates showed hypermucoviscosity and weak biofilm formation. In conclusion, 9% of string-positive, respectively 0.69% of all K. pneumoniae isolates from routine were defined as putative hypervirulent. MacConkey agar was the best medium for hvKp screening.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PYOGENIC LIVER-ABSCESS; VIRULENCE DETERMINANT; EMERGING DISEASE; SEROTYPE K1; IDENTIFICATION; MAGA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2024 05:40 |
| Last Modified: | 09 Apr 2024 05:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60648 |
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