Press, T. and Viale-Bouroncle, S. and Felthaus, O. and Gosau, M. and Morsczeck, C. (2015) EGR1 supports the osteogenic differentiation of dental stem cells. INTERNATIONAL ENDODONTIC JOURNAL, 48 (2). pp. 185-192. ISSN 0143-2885, 1365-2591
Full text not available from this repository. (Request a copy)Abstract
AimTo evaluate whether and how the transcription factor early growth response gene 1 (EGR1) affects the osteogenic differentiation of dental stem cells. MethodologyDental stem cells from apical papilla (SCAPs) and from the dental follicle (DFCs) were transfected with EGR1-specific siRNA or EGR-1 expression plasmid. Gene regulation was verified at protein level by Western blotting. The expression of the transcription factors distal-less homeobox 3 (DLX3), alkaline phosphatase (ALP) and bone morphogenetic protein 2 (BMP2), which are all regulators and markers of the osteogenic differentiation in dental stem cells, was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). To investigate mineralization, SCAP long-term cultures were stained with alizarin red after EGR1 over-expression. ResultsEGR1 was induced in SCAPs during osteogenic differentiation. DLX3 and bone morphogenetic protein 2 (BMP2) were up-regulated after EGR1 over-expression and down-regulated after EGR1 depletion. The expression of ALP was also down-regulated after EGR1 depletion. The over-expression of EGR1 in SCAPs promoted mineralization after osteogenic differentiation. ConclusionsEGR1 supported the osteogenic differentiation of dental stem cells by potentially regulating the expression of DLX3 and BMP2.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCRIPTION FACTOR; OSTEOBLAST DIFFERENTIATION; GENE-EXPRESSION; IN-VITRO; SIGNAL-TRANSDUCTION; PRECURSOR CELLS; APICAL PAPILLA; BONE-FORMATION; TISSUE-REPAIR; GROWTH; bone morphogenetic protein 2; dental follicle precursor cells; distal-less homeobox 3; early growth response gene 1; stem cells from apical papilla |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jul 2019 09:03 |
| Last Modified: | 25 Jul 2019 09:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6066 |
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