Wang, Han and Stehr, Antonia Margarethe and Singh, Jeeshan and Zlatar, Leticija and Hartmann, Arndt and Evert, Katja and Naschberger, Elisabeth and von Stillfried, Saskia and Boor, Peter and Munoz, Luis E. E. and Knopf, Jasmin and Stuerzl, Michael and Herrmann, Martin (2023) Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (4): 4101. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
During inflammatory responses, neutrophils enter the sites of attack where they execute various defense mechanisms. They (I) phagocytose microorganisms, (II) degranulate to release cytokines, (III) recruit various immune cells by cell-type specific chemokines, (IV) secrete anti-microbials including lactoferrin, lysozyme, defensins and reactive oxygen species, and (V) release DNA as neutrophil extracellular traps (NETs). The latter originates from mitochondria as well as from decondensed nuclei. This is easily detected in cultured cells by staining of DNA with specific dyes. However, in tissues sections the very high fluorescence signals emitted from the condensed nuclear DNA hamper the detection of the widespread, extranuclear DNA of the NETs. In contrast, when we employ anti-DNA-IgM antibodies, they are unable to penetrate deep into the tightly packed DNA of the nucleus, and we observe a robust signal for the extended DNA patches of the NETs. To validate anti-DNA-IgM, we additionally stained the sections for the NET-markers histone H2B, myeloperoxidase, citrullinated histone H3, and neutrophil elastase. Altogether, we have described a fast one-step procedure for the detection of NETs in tissue sections, which provides new perspectives to characterize neutrophil-associated immune reactions in disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEUTROPHIL ELASTASE; RELEASE; DAPI; 4,6-DIAMIDINO-2-PHENYLINDOLE; NUCLEOSOMES; MECHANISMS; TRANSPORT; BINDING; extracellular DNA; NETs; anti-DNA-IgM |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2024 06:09 |
| Last Modified: | 09 Apr 2024 06:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60675 |
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