Cai, Hanyang and Huang, Youmei and Liu, Liping and Zhang, Man and Chai, Mengnan and Xi, Xinpeng and Aslam, Mohammad and Wang, Lulu and Ma, Suzhuo and Su, Han and Liu, Kaichuang and Tian, Yaru and Zhu, Wenhui and Qi, Jingang and Dresselhaus, Thomas and Qin, Yuan (2023) Signaling by the EPFL-ERECTA family coordinates female germline specification through the BZR1 family in Arabidopsis. PLANT CELL, 35 (5). pp. 1455-1473. ISSN 1040-4651, 1532-298X
Full text not available from this repository. (Request a copy)Abstract
In most flowering plants, the female germline is initiated in the subepidermal L2 layer of ovule primordia forming a single megaspore mother cell (MMC). How signaling from the L1 (epidermal) layer could contribute to the gene regulatory network (GRN) restricting MMC formation to a single cell is unclear. We show that EPIDERMAL PATTERNING FACTOR-like (EPFL) peptide ligands are expressed in the L1 layer, together with their ERECTA family (ERf) receptor kinases, to control female germline specification in Arabidopsis thaliana. EPFL-ERf dependent signaling restricts multiple subepidermal cells from acquiring MMC-like cell identity by activating the expression of the major brassinosteroid (BR) receptor kinase BRASSINOSTEROID INSENSITIVE 1 and the BR-responsive transcription factor BRASSINOZOLE RESISTANT 1 (BZR1). Additionally, BZR1 coordinates female germline specification by directly activating the expression of a nucleolar GTP-binding protein, NUCLEOSTEMIN-LIKE 1 (NSN1), which is expressed in early-stage ovules excluding the MMC. Mutants defective in this GRN form multiple MMCs resulting in a strong reduction of seed set. In conclusion, we uncovered a ligand/receptor-like kinase-mediated signaling pathway acting upstream and coordinating BR signaling via NSN1 to restrict MMC differentiation to a single subepidermal cell. A ligand/receptor-like kinase-mediated signaling pathway coordinates brassinosteroid signaling via the GTP-binding protein NUCLEOSTEMIN-LIKE 1 to restrict megaspore mother cell differentiation to a single subepidermal cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIGAND-RECEPTOR PAIRS; WILD-TYPE; INFLORESCENCE ARCHITECTURE; OVULE DEVELOPMENT; GENE-EXPRESSION; CELL FATE; KINASE; GROWTH; ACTIVATION; MEIOSIS; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2024 09:41 |
| Last Modified: | 09 Apr 2024 09:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60676 |
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