Preissler, Julia and Grosche, Antje and Lede, Vera and Le Duc, Diana and Kruegel, Katja and Matyash, Vitali and Szulzewsky, Frank and Kallendrusch, Sonja and Immig, Kerstin and Kettenmann, Helmut and Bechmann, Ingo and Schoeneberg, Torsten and Schulz, Angela (2015) Altered Microglial Phagocytosis in GPR34-Deficient Mice. GLIA, 63 (2). pp. 206-215. ISSN 0894-1491, 1098-1136
Full text not available from this repository. (Request a copy)Abstract
GPR34 is a G(i/o) protein-coupled receptor (GPCR) of the nucleotide receptor P2Y(12)-like group. This receptor is highly expressed in microglia, however, the functional relevance of GPR34 in these glial cells is unknown. Previous results suggested an impaired immune response in GPR34-deficient mice infected with Cryptococcus neoformans. Here we show that GPR34 deficiency results in morphological changes in retinal and cortical microglia. RNA sequencing analysis of microglia revealed a number of differentially expressed transcripts involved in cell motility and phagocytosis. We found no differences in microglial motility after entorhinal cortex lesion and in response to laser lesion. However, GPR34-deficient microglia showed reduced phagocytosis activity in both retina and acutely isolated cortical slices. Our study identifies GPR34 as an important signaling component controlling microglial function, morphology and phagocytosis. GLIA 2015;63:206-215
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-COUPLED RECEPTOR; MYELIN PHAGOCYTOSIS; P2Y(12) RECEPTORS; IN-VIVO; CELLS; IDENTIFICATION; ATP; ACTIVATION; ASTROCYTES; EXPRESSION; microglia; GPCR; OHSC; RNA sequencing; GPR34; phagocytosis; neurodegenerative disease |
| Divisions: | Medicine > Lehrstuhl für Humangenetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jul 2019 09:13 |
| Last Modified: | 25 Jul 2019 09:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6071 |
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