Wang, Qingqing and Hoene, Miriam and Hu, Chunxiu and Fritsche, Louise and Ahrends, Robert and Liebisch, Gerhard and Ekroos, Kim and Fritsche, Andreas and Birkenfeld, Andreas L. and Liu, Xinyu and Zhao, Xinjie and Li, Qi and Su, Benzhe and Peter, Andreas and Xu, Guowang and Lehmann, Rainer (2023) Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies. JOURNAL OF LIPID RESEARCH, 64 (6): 100378. ISSN 0022-2275, 1539-7262
Full text not available from this repository. (Request a copy)Abstract
Reliability, robustness, and interlaboratory comparability of quantitative measurements is critical for clinical lipidomics studies. Lipids' different ex vivo stability in blood bears the risk of misinterpretation of data. Clear recommendations for the process of blood sample collection are required. We studied by UHPLC-high resolution mass spectrometry, as part of the "Preanalytics interest group" of the International Lipidomics Society, the stability of 417 lipid species in EDTA whole blood after exposure to either 4 degrees C, 21 degrees C, or 30 degrees C at six different time points (0.5 h-24 h) to cover common daily routine conditions in clinical settings. In total, >800 samples were analyzed. 325 and 288 robust lipid species resisted 24 h exposure of EDTA whole blood to 21 degrees C or 30 degrees C, respectively. Most significant instabilities were detected for FA, LPE, and LPC. Based on our data, we recommend cooling whole blood at once and permanent. Plasma should be separated within 4 h, unless the focus is solely on robust lipids. Lists are provided to check the ex vivo (in)stability of distinct lipids and potential biomarkers of interest in whole blood. To conclude, our results contribute to the international efforts towards reliable and comparable clinical lipin the future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN PLASMA; METABOLOMICS; RISK; Clinical lipidomics; blood; sample collection; preanalytical; lipid stability |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Jan 2024 10:52 |
| Last Modified: | 30 Jan 2024 10:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60714 |
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