Aloba, B. K. and Kinnevey, P. M. and Monecke, S. and Brennan, G. I. and O'Connell, B. and Blomfeldt, A. and McManus, B. A. and Schneider-Brachert, W. and Tkadlec, J. and Ehricht, R. and Senok, A. and Bartels, M. D. and Coleman, David C. (2023) An emerging Panton-Valentine leukocidin-positive CC5-meticillin-resistant<i> Staphylococcus</i><i> aureus-IVc</i> clone recovered from hospital and community settings over a 17-year period from 12 countries investigated by whole-genome sequencing. JOURNAL OF HOSPITAL INFECTION, 132. pp. 8-19. ISSN 0195-6701, 1532-2939
Full text not available from this repository. (Request a copy)Abstract
Background: A novel Panton-Valentine leukocidin (PVL)-positive meticillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC)5-MRSA-IVc ('Sri Lankan' clone) was recently described from Sri Lanka. Similar isolates caused a recent Irish hospital outbreak. Aim: To investigate the international dissemination and diversity of PVL-positive CC5-MRSA-IVc isolates from hospital and community settings using whole-genome sequencing (WGS).Methods: Core-genome single nucleotide polymorphism (cgSNP) analysis, core-genome multi-locus sequence typing (cgMLST) and microarray-based detection of antimicrobial -resistance and virulence genes were used to investigate PVL-positive CC5-MRSA-IVc (N 1/4 214 including 46 'Sri Lankan' clone) from hospital and community settings in 12 countries over 17 years. Comparators included 29 PVL-positive and 23 PVL-negative CC5/ST5-MRSA-I/II/IVa/IVc/IVg/V.Results: Maximum-likelihood cgSNP analysis grouped 209/214 (97.7%) CC5-MRSA-IVc into Clade I; average of 110 cgSNPs between isolates. Clade III contained the five remaining CC5-MRSA-IVc; average of 92 cgSNPs between isolates. Clade II contained seven PVL-positive CC5-MRSA-IVa comparators, whereas the remaining 45 comparators formed an outlier group. Minimum-spanning cgMLST analysis revealed a comparably low average of 57 allelic differences between all CC5/ST5-MRSA-IVc. All 214 CC5/ST5-MRSA-IVc were iden-tified as 'Sri Lankan' clone, predominantly spa type t002 (186/214) with low population diversity and harboured a similar range of virulence genes and variable antimicrobial -resistance genes. All 214 Sri Lankan clone isolates and Clade II comparators harboured a 9616-bp chromosomal PVL-encoding phage remnant, suggesting both arose from a PVL-positive meticillin-susceptible ancestor. Over half of Sri Lankan clone isolates were from infections (142/214), and where detailed metadata were available (168/214), most were community associated (85/168). Conclusions: Stable chromosomal retention of pvl may facilitate Sri-Lankan clone dissemination. 2022 The Author(s). Published by Elsevier Ltd on behalf of The Healthcare Infection Society. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESISTANT STAPHYLOCOCCUS-AUREUS; INFECTIONS; VIRULENCE; IRELAND; STRAINS; SKIN; CC5-MRSA-IVc; Dissemination; Epidemiology; Phylogenomics; PVL; Sri Lankan clone |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2024 09:14 |
| Last Modified: | 09 Apr 2024 09:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60757 |
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