Yoshinaga, Marcos Y. and Gagen, Emma J. and Woermer, Lars and Broda, Nadine K. and Meador, Travis B. and Wendt, Jenny and Thomm, Michael and Hinrichs, Kai-Uwe (2015) Methanothermobacter thermautotrophicus modulates s membrane lipids in response to hydrogen and nutrient availability. FRONTIERS IN MICROBIOLOGY, 6: 5. ISSN 1664-302X,
Full text not available from this repository. (Request a copy)Abstract
Methanothermobacter thermautotrophicus strain AH is a model hydrogenotrophic methanogen, for which extensive biochemical information, including the complete genome sequence, is available. Nevertheless, at the cell membrane lipid level, little is known about the responses of this archaeon to environmental stimuli. In this study, the lipid composition of M. the rmautotrophicus was characterized to verify how this archaeon modulates its cell membrane components during growth phases and in response to hydrogen depletion and nutrient limitation (potassium and phosphate). As opposed to the higher abundance of phospholipids in the stationary phase of control experiments, cell membranes under nutrient, and energy stress were dominated by glycolipids that likely provided a more effective barrier against ion leakage. We also identified particular lipid regulatory mechanisms in M. thermautotrophicus, which included the accumulation of polyprenols under hydrogen-limited conditions and an increased content of sodiated adducts of lipids in nutrient-limited cells. These findings suggest that M. thermautotrophicus intensely modulates its cell membrane lipid composition to cope with energy and nutrient availability in dynamic environments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTACT POLAR LIPIDS; ARCHAEAL TETRAETHER LIPIDS; METHANOBACTERIUM-THERMOAUTOTROPHICUM; MASS-SPECTROMETRY; THERMOPLASMA-ACIDOPHILUM; GROWTH TEMPERATURE; BIOSYNTHESIS; PHASE; SEDIMENTS; PATTERNS; archaea; stress response; polar lipids; diether; tetraether |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jul 2019 11:13 |
| Last Modified: | 25 Jul 2019 11:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6082 |
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