Esser, Sarah P. and Rahlff, Janina and Zhao, Weishu and Predl, Michael and Plewka, Julia and Sures, Katharina and Wimmer, Franziska and Lee, Janey and Adam, Panagiotis S. and McGonigle, Julia and Turzynski, Victoria and Banas, Indra and Schwank, Katrin and Krupovic, Mart and Bornemann, Till L. V. and Figueroa-Gonzalez, Perla Abigail and Jarett, Jessica and Rattei, Thomas and Amano, Yuki and Blaby, Ian K. and Cheng, Jan-Fang and Brazelton, William J. and Beisel, Chase L. and Woyke, Tanja and Zhang, Ying and Probst, Alexander J. (2023) A predicted CRISPR-mediated symbiosis between uncultivated archaea. NATURE MICROBIOLOGY, 8 (9). 1619-+. ISSN 2058-5276,
Full text not available from this repository. (Request a copy)Abstract
CRISPR spacers in DPANN archaea target putative essential genes in their episymbionts and could be a widespread occurrence across diverse archaeal lineages. CRISPR-Cas systems defend prokaryotic cells from invasive DNA of viruses, plasmids and other mobile genetic elements. Here, we show using metagenomics, metatranscriptomics and single-cell genomics that CRISPR systems of widespread, uncultivated archaea can also target chromosomal DNA of archaeal episymbionts of the DPANN superphylum. Using meta-omics datasets from Crystal Geyser and Horonobe Underground Research Laboratory, we find that CRISPR spacers of the hosts Candidatus Altiarchaeum crystalense and Ca. A. horonobense, respectively, match putative essential genes in their episymbionts' genomes of the genus Ca. Huberiarchaeum and that some of these spacers are expressed in situ. Metabolic interaction modelling also reveals complementation between host-episymbiont systems, on the basis of which we propose that episymbionts are either parasitic or mutualistic depending on the genotype of the host. By expanding our analysis to 7,012 archaeal genomes, we suggest that CRISPR-Cas targeting of genomes associated with symbiotic archaea evolved independently in various archaeal lineages.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EVOLUTIONARY CLASSIFICATION; IGNICOCCUS-HOSPITALIS; CAS SYSTEMS; CD-HIT; ALIGNMENT; ACCURATE; BACTERIA; PHYLOGENY; INSIGHTS; GENOMES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Apr 2024 14:24 |
| Last Modified: | 18 Apr 2024 14:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/60879 |
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