Kanageswaran, Ninthujah and Demond, Marilen and Nagel, Maximilian and Schreiner, Benjamin S. P. and Baumgart, Sabrina and Scholz, Paul and Altmueller, Janine and Becker, Christian and Doerner, Julia F. and Conrad, Heike and Oberland, Sonja and Wetzel, Christian H. and Neuhaus, Eva M. and Hatt, Hanns and Gisselmann, Guenter (2015) Deep Sequencing of the Murine Olfactory Receptor Neuron Transcriptome. PLOS ONE, 10 (1): e0113170. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
The ability of animals to sense and differentiate among thousands of odorants relies on a large set of olfactory receptors (OR) and a multitude of accessory proteins within the olfactory epithelium (OE). ORs and related signaling mechanisms have been the subject of intensive studies over the past years, but our knowledge regarding olfactory processing remains limited. The recent development of next generation sequencing (NGS) techniques encouraged us to assess the transcriptome of the murine OE. We analyzed RNA from OEs of female and male adult mice and from fluorescence-activated cell sorting (FACS)-sorted olfactory receptor neurons (ORNs) obtained from transgenic OMP-GFP mice. The Illumina RNA-Seq protocol was utilized to generate up to 86 million reads per transcriptome. In OE samples, nearly all OR and trace amine-associated receptor (TAAR) genes involved in the perception of volatile amines were detectably expressed. Other genes known to participate in olfactory signaling pathways were among the 200 genes with the highest expression levels in the OE. To identify OE-specific genes, we compared olfactory neuron expression profiles with RNA-Seq transcriptome data from different murine tissues. By analyzing different transcript classes, we detected the expression of non-olfactory GPCRs in ORNs and established an expression ranking for GPCRs detected in the OE. We also identified other previously undescribed membrane proteins as potential new players in olfaction. The quantitative and comprehensive transcriptome data provide a virtually complete catalogue of genes expressed in the OE and present a useful tool to uncover candidate genes involved in, for example, olfactory signaling, OR trafficking and recycling, and proliferation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; EXCHANGER REGULATORY FACTOR; MAMMALIAN ODORANT RECEPTORS; PROTEIN-COUPLED RECEPTORS; NUCLEOTIDE-GATED CHANNELS; BETA-GAMMA-SUBUNITS; G-ALPHA-OLF; SENSORY NEURONS; SIGNAL-TRANSDUCTION; RNA-SEQ; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Psychiatrie und Psychotherapie > Molekulare Neurowissenscahften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jul 2019 11:49 |
| Last Modified: | 25 Jul 2019 11:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6097 |
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