Richter, C. and Schneider, C. and Quick, M. T. and Volz, P. and Mahrwald, R. and Hughes, J. and Dick, B. and Alexiev, U. and Ernsting, N. P. (2015) Dual-fluorescence pH probe for bio-labelling. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 17 (45). pp. 30590-30597. ISSN 1463-9076, 1463-9084
Full text not available from this repository. (Request a copy)Abstract
Although seminaphtorhodafluor (SNARF) dyes are already widely used to measure pH in cells and at biofilms, their synthesis has low yield and results in an unspecific position of a carboxy-group. The separation of 5'-and 6'-carboxy-SNARF reveals a pK(a) difference of 0.15, calling into question pH measurements with the (commercially available) mixture. Here we replace the bulky external dicarboxyphenyl ring with a propionate group and evaluate the spectral properties of the new derivative. Proceeding to the ethyl-iodoacetamide, covalent linkage to cysteine protein sites is achieved efficiently as shown with a cyanobacterial phytochrome, extending the scarce application of SNARF in bio-labelling in the current literature. Application in fluorescence lifetime imaging is demonstrated both with the lifetime-based and ratiometric-yield method.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REAL-TIME; CELLS; BACTERIORHODOPSIN; SPECTROSCOPY; PHOTOPHYSICS; MOLECULE; LACTONE; SURFACE; DYES; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jul 2019 09:57 |
| Last Modified: | 26 Jul 2019 09:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6156 |
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