Reuss, Julia and Rachel, Reinhard and Kaempfer, Peter and Rabenstein, Andreas and Kuever, Jan and Droege, Stefan and Koenig, Helmut (2015) Isolation of methanotrophic bacteria from termite gut. MICROBIOLOGICAL RESEARCH, 179. pp. 29-37. ISSN 0944-5013,
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The guts of termites feature suitable conditions for methane oxidizing bacteria (MOB) with their permanent production of CH4 and constant supply of O-2 via tracheae. In this study, we have isolated MOB from the gut contents of the termites Incisitermes marginipennis, Mastotermes darwiniensis, and Neotermes castaneus for the first time. The existence of MOB was indicated by detecting pmoA, the gene for the particulate methane monooxygenase, in the DNA of gut contents. Fluorescence in situ hybridization and quantitative real-time polymerase chain reaction supported those findings. The MOB cell titer was determined to be 10(2)-10(3) per gut. Analyses of the 16S rDNA from isolates indicated close similarity to the genus Methyiocystis. After various physiological tests and fingerprinting methods, no exact match to a known species was obtained, indicating the isolation of new MOB species. However, MALDI-TOF MS analyses revealed a close relationship to Methylocystis bryophila and Methylocystis parvus. (C) 2015 Elsevier GmbH. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 16S RIBOSOMAL-RNA; SP NOV.; MASTOTERMES-DARWINIENSIS; RETICULITERMES-SPERATUS; ATMOSPHERIC METHANE; TARGETED PROBES; IDENTIFICATION; SOIL; GROUNDWATER; ARCHAEA; Methane oxidizing bacteria; Microflora of termites; Isolation of MOB; Methylocystis; pmoA |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2019 08:12 |
| Last Modified: | 29 Jul 2019 08:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6185 |
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