Lippert, Elisabeth and Stieber-Gunckel, Manuela and Dunger, Nadja and Falk, Werner and Obermeier, Florian and Kunst, Claudia (2015) Galectin-3 Modulates Experimental Colitis. DIGESTION, 92 (1). pp. 45-53. ISSN 0012-2823, 1421-9867
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Background: We recently identified galectin-3 (gal-3) as a new and strong fibroblast activator produced by colonic epithelial cells. Very little is known about the influence of gal-3 in inflammatory bowel disease. We, therefore, investigated the impact of gal-3 on dextran sodium sulfate (DSS)-induced colitis in a mouse model. Methods: Colonic lamina propria fibroblasts of healthy controls were used for co-incubation studies of gal-3 with gal-1, TGF-beta 1, IFN gamma, IL-4 and IL-10. Acute and chronic DSS colitis was induced by 3% DSS in drinking water in female Balb/c mice weighing 20-22 g. Recombinant gal-3 was expressed by the pET vector system and used for a 5-day treatment in different concentrations intraperitoneally. The distal third of the colon was used for histologic analysis. Colonic cytokine expression was determined by quantitative RT-PCR. Results: In vitro, gal-3 induced IL-8 secretion was significantly reduced by co-incubation with IL-10 (5 ng/ml) and IL-4 (10 ng/ml). Acute DSS-induced colitis was ameliorated by gal-3 treatment as indicated by increased colonic length and reduced weight loss compared to that of controls. In acute and chronic colitis, gal-3 treatment resulted in a significant suppression of colonic IL-6. Conclusion: Gal-3 significantly reduces inflammation in acute and chronic DSS colitis in mice indicating a potential role in intestinal inflammation. (C) 2015 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ANTI-INTERLEUKIN-6 RECEPTOR ANTIBODY; CROHNS-DISEASE; DENDRITIC CELL; INFLAMMATORY RESPONSES; MESSENGER-RNA; ACTIVATION; EXPRESSION; APOPTOSIS; MOLECULE; BINDING; Colitis; Crohn's disease; Inflammation; Inflammatory bowel disease; Ulcerative colitis; Galectin-3 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Jul 2019 14:00 |
| Last Modified: | 29 Jul 2019 14:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6214 |
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