Wegener, Joachim (2015) Cell-Based Microarrays for In Vitro Toxicology. In: UNSPECIFIED Annual Review of Analytical Chemistry,, 8 . ANNUAL REVIEWS, PALO ALTO, pp. 335-358. ISBN 978-0-8243-4408-5
Full text not available from this repository. (Request a copy)Abstract
DNA/RNA and protein microarrays have proven their outstanding bioanalytical performance throughout the past decades, given the unprecedented level of parallelization by which molecular recognition assays can be performed and analyzed. Cell microarrays (CMAs) make use of similar construction principles. They are applied to profile a given cell population with respect to the expression of specific molecular markers and also to measure functional cell responses to drugs and chemicals. This review focuses on the use of cell-based microarrays for assessing the cytotoxicity of drugs, toxins, or chemicals in general. It also summarizes CMA construction principles with respect to the cell types that are used for such microarrays, the readout parameters to assess toxicity, and the various formats that have been established and applied. The review ends with a critical comparison of CMAs and well-established microtiter plate (MTP) approaches.
| Item Type: | Book Section |
|---|---|
| Uncontrolled Keywords: | SURFACE-PLASMON RESONANCE; DIGITAL HOLOGRAPHIC MICROSCOPY; PROTEIN-COUPLED RECEPTORS; IMPEDANCE SENSING ECIS; HIGH-THROUGHPUT; CELLCARD(TM) SYSTEM; MOLECULAR-MECHANISMS; MAMMALIAN-CELLS; CULTURE SYSTEMS; ARRAYS; cell microarrays; cell-based microarrays; in vitro toxicology; cell-based assays; cell-based biosensors; cytotoxicity |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2020 09:10 |
| Last Modified: | 22 Jul 2020 09:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6228 |
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