Lang, Siegmund and Herrmann, Marietta and Pfeifer, Christian and Brockhoff, Gero and Zellner, Johannes and Nerlich, Michael and Angele, Peter and Prantl, Lukas and Gehmert, Sebastian and Loibl, Markus (2017) Leukocyte-reduced platelet-rich plasma stimulates the in vitro proliferation of adipose-tissue derived mesenchymal stem cells depending on PDGF signaling. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, 67 (2). pp. 183-196. ISSN 1386-0291, 1875-8622
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements. OBJECTIVE: The aim of this study was to investigate PRP-triggered signaling in adipose derived mesenchymal stem cells (ASCs). METHODS: PRP was obtained from 4 male patients. We incubated ASCs in alpha-MEM with different Platelet derived growth factor (PDGF) subtypes or 10% or 20% pooled PRP or 20% fetal calf serum (FCS) prior to determination of the S-phase fraction (SPF). To investigate the influence of PDGF signaling on ASCs, PDGF receptor beta inhibitor was added, and protein expression of ASCs was measured. RESULTS: ASCs exposed to 20% PRP, PDGF-AB and -BB demonstrated significant higher SPF in comparison to PDGF-AA and 20% FCS after 48 hours (all P < 0.05). PDGF receptor beta inhibition diminished the PRP-induced SPF increase of ASCs significantly after 48 hours (P < 0.01). ASCs with PDGF receptor beta inhibition showed significant higher PDGF receptor beta and significant lower c-MYC expression compared to untreated cells in presence of 20% PRP after 48 hours (both P < 0.05). CONCLUSIONS: The proliferation promoting effect of PRP on ASCs is mediated by PDGF signaling and is associated with c-MYC overexpression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR CONCENTRATIONS; FETAL BOVINE SERUM; SEPARATION SYSTEMS; STROMAL CELLS; MYC-ONCOGENE; BONE-MARROW; CALF SERUM; DIFFERENTIATION; EXPRESSION; EXPANSION; Platelet-rich Plasma; mesenchymal stem cells; PDGF receptor beta inhibition; proliferation; cell culture |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Unfallchirurgie Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 26 Feb 2019 06:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/624 |
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