Zupke, Oliver and Distler, Eva and Juerchott, Anna and Paiphansiri, Umaporn and Dass, Martin and Thomas, Simone and Hartwig, Udo F. and Theobald, Matthias and Landfester, Katharina and Mailaender, Volker and Herr, Wolfgang (2015) Nanoparticles and antigen-specific T-cell therapeutics: a comprehensive study on uptake and release. NANOMEDICINE, 10 (7). pp. 1063-1076. ISSN 1743-5889, 1748-6963
Full text not available from this repository. (Request a copy)Abstract
Aim: T lymphocytes are used as cellular therapeutics in many disease entities including cancer. We investigated the uptake and retention of nanoparticles (NPs) by these nonphagocytic cells. Materials & methods: Uptake, release and toxicity of various polymeric NPpreparations were analyzed by flow cytometry, confocal laser scanning microscopy and transmission electron microscopy. T-cell effector functions were measured using IFN-gamma-ELISPOT and 51Chromium-release assays. Results: Aminofunctionalized NPs were efficiently ingested by antigen-specific T cells without adversely influencing effector functions. NPs were stored in membrane-surrounded vesicles, with major proportions released extracellularly during 24 h. Conclusion: Amino-functionalized polymeric NPs are efficiently taken up by human T cells and could be used to design nanocarriers for direct access and manipulation of antigen-specific T cells in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE DELIVERY; PARTICLES; TRANSPLANTATION; LYMPHOCYTES; DRUG; MINIEMULSION; MECHANISM; RESPONSES; SUBSETS; CANCER; cell imaging; cellular therapy; drug delivery; endocytosis; leukemia; nanoparticles; NP release; T lymphocytes; tumor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jul 2019 08:59 |
| Last Modified: | 31 Jul 2019 08:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6251 |
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