Fenzl, Christoph and Genslein, Christa and Zoepfl, Alexander and Baeumner, Antje J. and Hirsch, Thomas (2015) A photonic crystal based sensing scheme for acetylcholine and acetylcholinesterase inhibitors. JOURNAL OF MATERIALS CHEMISTRY B, 3 (10). pp. 2089-2095. ISSN 2050-750X, 2050-7518
Full text not available from this repository. (Request a copy)Abstract
We present a new scheme for sensing biomolecules by combining an enzyme hydrogel with a photonic crystal hydrogel layer that responds to ionic strength and pH changes. We demonstrate this unique combination by successfully detecting acetylcholine (ACh) and acetylcholinesterase (AChE) inhibitors. Specifically, the sandwich assembly is composed of layers of photonic crystals and a polyacrylamide hydrogel functionalized with AChE. The photonic crystal film has a red color and turns dark purple within 2-6 minutes of the enzymatic reaction upon analyte addition. This 3D photonic crystal sensor responds to acetylcholine in the 1 nM to 10 mu M concentration range (which includes the relevant range of ACh concentrations in human body fluids). Michaelis-Menten kinetics of the enzyme were determined which correlated well with literature data demonstrating the uninhibited reactivity of the immobilized enzyme. Furthermore, the presence of the acetylcholinesterase inhibitor neostigmine at concentrations as low as 1 fM was demonstrated, which is even below the necessary detection limit for clinical diagnostics. We suggest that this novel concept will find its application in clinical diagnostics, for pesticide and nerve agent detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IONIC-STRENGTH; HYDROGEL; SENSORS; ENZYME; ARRAY; WATER; PH; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Aug 2019 11:47 |
| Last Modified: | 01 Aug 2019 11:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6283 |
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