Mueller, Andreas and Koenig, Burkhard (2015) Preparation of luminescent chemosensors by post-functionalization of vesicle surfaces. ORGANIC & BIOMOLECULAR CHEMISTRY, 13 (6). pp. 1690-1699. ISSN 1477-0520, 1477-0539
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Surface-reactive luminescent vesicles were prepared by self-assembly of phospholipids, amphiphilic maleimides and fluorophors in aqueous solution. Those preformed liposomes were functionalized with various thiolated receptor units using a thiol-click reaction. As recognition elements, a bis-Zn2+-cyclen derivative for the detection of phosphate moieties or a DNA aptamer for the specific binding of the antibiotic ampicillin were utilized. A FRET-based assay revealed the close spatial proximity of the membrane-embedded dansyl molecules with the subsequently immobilized thiols, which is the origin for the signaling mechanism of the obtained vesicular sensors. Those receptor-functionalized liposomes indicate the binding of the targets to their surface by changes of the fluorescence emission properties of the membrane co-embedded carboxyfluorescein dyes. The post-functionalization concept can also be used for molecular imprinting on vesicle surfaces. The template-guided patterning of receptors based on bis-Zn2+- cyclen resulted in fluorescent sensors suitable for the specific recognition of a bivalent peptide.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MOLECULAR RECOGNITION; BINDING-SITES; LIPOSOMES; MALEIMIDE; COMPLEX; THIOLS; NANOPARTICLES; FLUORESCENT; APTASENSOR; REACTIVITY; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Aug 2019 08:34 |
| Last Modified: | 02 Aug 2019 08:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6302 |
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