Giannone, Richard J. and Wurch, Louie L. and Heimerl, Thomas and Martin, Stanton and Yang, Zamin and Huber, Harald and Rachel, Reinhard and Hettich, Robert L. and Podar, Mircea (2015) Life on the edge: functional genomic response of Ignicoccus hospitalis to the presence of Nanoarchaeum equitans. ISME JOURNAL, 9 (1). pp. 101-114. ISSN 1751-7362, 1751-7370
Full text not available from this repository. (Request a copy)Abstract
The marine hyperthermophilic crenarchaeon Ignicoccus hospitalis supports the propagation on its surface of Nanoarchaeum equitans, an evolutionarily enigmatic archaeon that resembles highly derived parasitic and symbiotic bacteria. The cellular and molecular mechanisms that enable this interarchaea relationship and the intimate physiologic consequences to I. hospitalis are unknown. Here, we used concerted proteomic and transcriptomic analyses to probe into the functional genomic response of I. hospitalis as N. equitans multiplies on its surface. The expression of over 97% of the genes was detected at mRNA level and over 80% of the predicted proteins were identified and their relative abundance measured by proteomics. These indicate that little, if any, of the host genomic information is silenced during growth in the laboratory. The primary response to N. equitans was at the membrane level, with increases in relative abundance of most protein complexes involved in energy generation as well as that of several transporters and proteins involved in cellular membrane stabilization. Similar upregulation was observed for genes and proteins involved in key metabolic steps controlling nitrogen and carbon metabolism, although the overall biosynthetic pathways were marginally impacted. Proliferation of N. equitans resulted, however, in selective downregulation of genes coding for transcription factors and replication and cell cycle control proteins as I. hospitalis shifted its physiology from its own cellular growth to that of its ectosymbiont/parasite. The combination of these multiomic approaches provided an unprecedented level of detail regarding the dynamics of this interspecies interaction, which is especially pertinent as these organisms are not genetically tractable.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PEPTIDE IDENTIFICATION; TRANSCRIPTOME ANALYSIS; ARCHAEAL EVOLUTION; OUTER-MEMBRANE; SP-NOV; INSIGHTS; PROTEOME; HOST; DIVERSITY; ANCESTOR; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Aug 2019 11:03 |
| Last Modified: | 02 Aug 2019 11:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6332 |
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