Lier, Constantin and Dejaco, Alexander and Kratzer, Alexander and Kees, Martin G. and Kees, Frieder and Dorn, Christoph (2024) Free serum concentrations of antibiotics determined by ultrafiltration: extensive evaluation of experimental variables. BIOANALYSIS, 16 (14). pp. 747-756. ISSN 1757-6180, 1757-6199
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Aim: To assess the impact of experimental conditions on free serum concentrations as determined by ultrafiltration and HPLC-DAD analysis in a wide range of antibiotics. Materials & methods: Relative centrifugation force (RCF), temperature, pH and buffer were varied and the results compared with the standard protocol (phosphate buffer pH 7.4, 37 degrees C, 1000 x g). Results: Generally, at 10,000 x g the unbound fraction (fu) decreased with increasing molecular weight, and was lower at 22 degrees C. In unbuffered serum, the fu of flucloxacillin or valproic acid was increased, that of basic or amphoteric drugs considerably decreased. Comparable results were obtained using phosphate or HEPES buffer except for drugs which form metal chelate complexes. Conclusion: Maintaining a physiological pH is more important than strictly maintaining body temperature.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BETA-LACTAM ANTIBIOTICS; PROTEIN-BINDING; HUMAN PLASMA; UNBOUND FRACTION; NONSPECIFIC-BINDING; FREE-THYROXINE; DRUGS; ALBUMIN; IMPACT; CEFTRIAXONE; anti-infectives; azole antimycotics; beta-lactams; HPLC-UV; plasma protein binding; therapeutic drug monitoring; unbound fraction; valproic acid |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert) Chemistry and Pharmacy > Institute of Pharmacy > Group Clinical Pharmacy (Dr. Dorn) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2025 06:45 |
| Last Modified: | 15 Jul 2025 06:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/63350 |
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