Plain, Allein and Knoedl, Laura and Tegtmeier, Ines and Bandulik, Sascha and Warth, Richard (2024) The ex vivo perfused mouse adrenal gland-a new model to study aldosterone secretion. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 476 (6). pp. 911-922. ISSN 0031-6768, 1432-2013
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Aldosterone is a steroid hormone that is important for maintaining the volume and ionic composition of extracellular fluids and is produced in the zona glomerulosa of the adrenal cortex. The basic mechanisms controlling aldosterone secretion are known. However, more detailed studies on the regulation of aldosterone secretion often fail due to the lack of suitable models: although secretion can be studied in cultured adrenocortical cells under defined conditions, the differentiation status of the cells is difficult to control and the complex anatomy of the adrenal cortex is lost. In living animals, the physiological context is intact, but the influences are manifold and the examination conditions cannot be sufficiently controlled. One method that closes the gap between cell models and studies in living animals is the isolated perfused adrenal gland. In the past, this method has provided important data on the pathophysiology of adrenal glands from larger animals, but the technique was not used in mice. Here, we developed a method for isolation and perfusion of the mouse adrenal gland to study aldosterone secretion. This technique preserves the complex anatomical and functional context of the mouse adrenal cortex, to ensure defined experimental conditions and to minimize extra-adrenal influences. Initial series of experiments with the ex vivo perfused mouse adrenal gland show that this model offers the possibility for unique insights into pathophysiological regulatory principles and is suitable for the use of genetically modified mouse models.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POTASSIUM CHANNELS; LOW-RENIN; OSMOLALITY; PATHOPHYSIOLOGY; CALCIUM; GENE; MICE; Adrenal cortex; Mouse models; Aldosteronism; Secretion; Pathophysiology |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Jul 2025 06:13 |
| Last Modified: | 18 Jul 2025 06:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/63440 |
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