Starke, Charlotte and Betz, Hannah and Hickmann, Linda and Lachmann, Peter and Neubauer, Bjoern and Kopp, Jeffrey B. and Sequeira-Lopez, Maria Luisa S. and Gomez, R. Ariel and Hohenstein, Bernd and Todorov, Vladimir T. and Hugo, Christian P. M. (2015) Renin Lineage Cells Repopulate the Glomerular Mesangium after Injury. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 26 (1). pp. 48-54. ISSN 1046-6673, 1533-3450
Full text not available from this repository. (Request a copy)Abstract
Mesangial cell injury has a major role in many CKDs. Because renin-positive precursor cells give rise to mesangial cells during nephrogenesis, this study tested the hypothesis that the same phenomenon contributes to glomerular regeneration after murine experimental mesangial injury. Mesangiolysis was induced by administration of an anti-mesangial cell serum in combination with LPS. In enhanced green fluorescent protein-reportermice with constitutively labeled renin lineage cells, the size of the enhanced green fluorescent protein-positive area in the glomerular tufts increased after mesangial injury. Furthermore, we generated a novel Tet-on inducible triple-transgenic LacZ reporter line that allowed selective labeling of renin cells along renal afferent arterioles of adult mice. Although no intraglomerular LacZ expression was detected in healthy mice, about two-thirds of the glomerular tufts became LacZ positive during the regenerative phase after severe mesangial injury. Intraglomerular renin descendant LacZ-expressing cells colocalized with mesangial cell markers alpha 8-integrin and PDGF receptor-beta but not with endothelial, podocyte, or parietal epithelial cell markers. In contrast with LacZ-positive cells in the afferent arterioles, LacZ-positive cells in the glomerular tuft did not express renin. These data demonstrate that extraglomerular renin lineage cells represent a major source of repopulating cells for reconstitution of the intraglomerular mesangium after injury.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXPRESSION; MICE; GLOMERULONEPHRITIS; NEPHRITIS; PHENOTYPE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Aug 2019 11:53 |
| Last Modified: | 02 Aug 2019 11:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/6349 |
Actions (login required)
 |
View Item |
