Morgenstern, Eva and Molthof, Carolin and Schwartz, Uwe and Graf, Johannes and Bruckmann, Astrid and Hombach, Sonja and Kretz, Markus (2024) lncRNA LINC00941 modulates MTA2/NuRD occupancy to suppress premature human epidermal differentiation. LIFE SCIENCE ALLIANCE, 7 (7): e202302475. ISSN , 2575-1077
Full text not available from this repository. (Request a copy)Abstract
Numerous long non-coding RNAs (lncRNAs) were shown to have a functional impact on cellular processes such as human epidermal homeostasis. However, the mechanism of action for many lncRNAs remains unclear to date. Here, we report that lncRNA LINC00941 regulates keratinocyte differentiation on an epigenetic level through association with the NuRD complex, one of the major chromatin remodelers in cells. We find that LINC00941 interacts with NuRD-associated MTA2 and CHD4 in human primary keratinocytes. LINC00941 perturbation changes MTA2/NuRD occupancy at bivalent chromatin domains in close proximity to transcriptional regulator genes, including the EGR3 gene coding for a transcription factor regulating epidermal differentiation. Notably, LINC00941 depletion resulted in reduced NuRD occupancy at the EGR3 gene locus, increased EGR3 expression in human primary keratinocytes, and increased abundance of EGR3regulated epidermal differentiation genes in cells and human organotypic epidermal tissues. Our results therefore indicate a role of LINC00941/NuRD in repressing EGR3 expression in nondifferentiated keratinocytes, consequentially preventing premature differentiation of human epidermal tissues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NURD-MEDIATED DEACETYLATION; STEM-CELLS; COMPLEX; EXPRESSION; GENES; TRANSCRIPTION; PLURIPOTENCY; H3K27; P63; ACT; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jul 2025 11:48 |
| Last Modified: | 15 Jul 2025 11:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/63508 |
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