Maerkl, Susanne and Przybilla, Frederic and Rachel, Reinhard and Hirsch, Thomas and Keller, Max and Witzgall, Ralph and Mely, Yves and Wegener, Joachim (2024) Impact of surface chemistry of upconversion nanoparticles on time-dependent cytotoxicity in non-cancerous epithelial cells. SCIENTIFIC REPORTS, 14 (1): 30610. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
The application of upconversion nanoparticles (UCNPs) for cell and tissue analysis requires a comprehensive understanding of their interactions with biological entities to prevent toxicity or harmful effects. Whereas most studies focus on cancer cells, this work addresses non-cancerous cells with their regular in vitro physiology. Since it is generally accepted that surface chemistry largely determines biocompatibility in general and uptake of nanomaterials in particular, two bilayer surface coatings with different surface shielding properties have been studied: (i) a phospholipid bilayer membrane (PLM) and (ii) an amphiphilic polymer (AP). Both surface modifications are applied to (12-33) nm core-shell UCNPs NaYF4(Yb, Er)@NaYF4, ensuring colloidal stability in biological media. The impact of UCNPs@AP and UCNPs@PLM on non-cancerous epithelial-like kidney cells in vitro was found to differ significantly. UCNPs@PLM did not exhibit any measurable effect on cell physiology, even with prolonged exposure. In contrast, UCNPs@AP caused changes in cell morphology and induced cell-death after approximately 30 h. These variations in toxicity are attributed to the distinct chemical stability of these particles, which likely influences their intracellular disintegration.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UPCONVERTING NANOPARTICLES; CELLULAR UPTAKE; GOLD NANOPARTICLES; PROTEIN CORONA; TOXICITY; DISINTEGRATION; STABILITY; COATINGS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jul 2025 06:06 |
| Last Modified: | 22 Jul 2025 06:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/63598 |
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