Schreiber, Laura and Ghimire, Sakhila and Hiergeist, Andreas and Renner, Kathrin and Althammer, Michael and Babl, Nathalie and Peuker, Alice and Schoenhammer, Gabriele and Hippe, Katrin and Gessner, Andre and Albrecht, Christin and Pielmeier, Fransziska and Buettner-Herold, Maike and Bruns, Heiko and Hoffmann, Petra and Herr, Wolfgang and Holler, Ernst and Peter, Katrin and Kreutz, Marina and Matos, Carina (2024) Strain specific differences in vitamin D3 response: impact on gut homeostasis. FRONTIERS IN IMMUNOLOGY, 15: 1347835. ISSN 1664-3224,
Full text not available from this repository. (Request a copy)Abstract
Vitamin D3 regulates a variety of biological processes irrespective of its well-known importance for calcium metabolism. Epidemiological and animal studies indicate a role in immune regulation, intestinal barrier function and microbiome diversity. Here, we analyzed the impact of different vitamin D3- containing diets on C57BL/6 and BALB/c mice, with a particular focus on gut homeostasis and also investigated effects on immune cells in vitro. Weak regulatory effects were detected on murine T cells. By trend, the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 suppressed IFN, GM-CSF and IL-10 cytokine secretion in T cells of C57BL/6 but not BALB/c mice, respectively. Using different vitamin D3-fortified diets, we found a tissue-specific enrichment of mainly CD11b+ myeloid cells but not T cells in both mouse strains e.g. in spleen and Peyer's Patches. Mucin Reg3 gamma and Batf expression, as well as important proteins for gut homeostasis, were significantly suppressed in the small intestine of C57BL76 but not BALB/c mice fed with a high-vitamin D3 containing diet. Differences between both mouse stains were not completely explained by differences in vitamin D3 receptor expression which was strongly expressed in epithelial cells of both strains. Finally, we analyzed gut microbiome and again an impact of vitamin D3 was detected in C57BL76 but not BALB/c. Our data suggest strain-specific differences in vitamin D3 responsiveness under steady state conditions which may have important implications when choosing a murine disease model to study vitamin D3 effects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONIC MUCUS BARRIER; D-RECEPTOR; 1,25-DIHYDROXYVITAMIN D-3; DENDRITIC CELLS; DYSBIOSIS; DEGRADES; vitamin D3; barrier function; intestinal; C57BL/6; BALB/c |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Jul 2025 07:24 |
| Last Modified: | 17 Jul 2025 07:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/63628 |
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