The Influence of CLSM Magnification on the Measured Roughness of Differently Prepared Dental Materials

Rosentritt, Martin and Schmutzler, Anne and Hahnel, Sebastian and Kurzendorfer-Brose, Laura (2024) The Influence of CLSM Magnification on the Measured Roughness of Differently Prepared Dental Materials. MATERIALS, 17 (23): 5954. ISSN , 1996-1944

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Abstract

This in vitro study investigated how varying magnifications (5x, 10x, 20x, and 50x) using a confocal laser scanning microscope (CLSM) influence the measured surface roughness parameters, R-a/S-a and R-z/S-z, of various materials with two surface treatments. Cylindrical specimens (d approximate to 8 mm, h approximate to 3 mm, n = 10) from titanium, zirconia, glass-ceramic, denture base material, and composite underwent diamond treatment (80 mu m; wet) and polishing (#4000; wet; Tegramin-25, Struers, G). The surface roughness parameters (R-a/S-a, R-z/S-z) were measured with a CLSM (VK-100, Keyence, J) at 5x, 10x, 20x, and 50x magnifications. Line roughness (R-a/R-z) was measured along a 1000 mu m distance in three parallel lines, while area roughness (S-a/S-z) was evaluated over a 2500 mu m x 1900 mu m area. The statistical analysis included ANOVA, the Bonferroni post hoc test, and Pearson correlation (SPSS 29, IBM, USA; alpha = 0.05). R-a/S-a and R-z/S-z showed significant differences (p <= 0.001, ANOVA) across magnifications, with values decreasing as magnification increased, highest at 5x and lowest at 50x. Titanium, zirconia, and glass-ceramic showed significant measured roughness values from 5x to 50x. Denture base material and composite had lower measured roughness values, especially after polishing. Line and area roughness varied significantly, indicating that magnification affects measured values. Standardizing magnifications is essential to ensure comparability between studies. A 50x magnification captures more detailed profile information while masking larger defects.

Item Type: Article
Uncontrolled Keywords: SURFACE-ROUGHNESS; FLEXURAL STRENGTH; COLOR-CHANGE; MICROSCOPY; surface roughness; R-a/S-a; R-z/S-z; confocal laser scanning microscopy; CLSM; magnification; dental materials
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Zahnärztliche Prothetik
Depositing User: Dr. Gernot Deinzer
Date Deposited: 24 Jul 2025 13:09
Last Modified: 24 Jul 2025 13:09
URI: https://pred.uni-regensburg.de/id/eprint/63719

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