Zhao, You and Mannala, Gopala Krishna and Youf, Raphaelle and Rupp, Markus and Alt, Volker and Riool, Martijn (2024) Development of a Galleria mellonella Infection Model to Evaluate the Efficacy of Antibiotic-Loaded Polymethyl Methacrylate (PMMA) Bone Cement. ANTIBIOTICS-BASEL, 13 (8): 692. ISSN 2079-6382,
Full text not available from this repository. (Request a copy)Abstract
Prosthetic joint infections (PJIs) can have disastrous consequences for patient health, including removal of the device, and placement of cemented implants is often required during surgery to eradicate PJIs. In translational research, in vivo models are widely used to assess the biocompatibility and antimicrobial efficacy of antimicrobial coatings and compounds. Here, we aim to utilize Galleria mellonella implant infection models to assess the antimicrobial activity of antibiotic-loaded bone cement (ALBC) implants. Therefore, we used commercially available bone cement loaded with either gentamicin alone (PALACOS R+G) or with a combination of gentamicin and vancomycin (COPAL G+V), compared to bone cement without antibiotics (PALACOS R). Firstly, the in vitro antimicrobial activity of ALBC was determined against Staphylococcus aureus. Next, the efficacy of ALBC implants was analyzed in both the G. mellonella hematogenous and early-stage biofilm implant infection model, by monitoring the survival of larvae over time. After 24 h, the number of bacteria on the implant surface and in the tissue was determined. Larvae receiving dual-loaded COPAL G+V implants showed higher survival rates compared to implants loaded with only gentamicin (PALACOS R+G) and the control implants without antibiotics (PALACOS R). In conclusion, G. mellonella larvae infection models with antibiotic-loaded bone cements are an excellent option to study (novel) antimicrobial approaches.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BIOFILM FORMATION; GENTAMICIN; HEMIARTHROPLASTY; PROPHYLAXIS; MANAGEMENT; DISEASES; REDUCE; DRUGS; HIP; antibiotic-loaded bone cement; Galleria mellonella; prosthetic joint infection; Staphylococcus aureus; biofilm; in vivo |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jan 2026 05:52 |
| Last Modified: | 20 Jan 2026 05:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/63938 |
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