Sanna, Franziska Christina and Benesova, Iva and Pervan, Philip and Krenz, Adriana and Wurzel, Alexander and Lohmayer, Robert and Muehlbauer, Jasmin and Woellner, Amelie and Koehl, Nina and Menevse, Ayse Nur and Stamova, Slava and Volpin, Valentina and Beckhove, Philipp and Xydia, Maria (2024) IL-2 and TCR stimulation induce expression and secretion of IL-32β by human T cells. FRONTIERS IN IMMUNOLOGY, 15: 1437224. ISSN 1664-3224,
Full text not available from this repository. (Request a copy)Abstract
IL-32 expression is important for pathogen clearance but detrimental in chronic inflammation, autoimmunity, and cancer. T cells are major IL-32 producers in these diseases and key mediators of pathogen and tumor elimination but also autoimmune destruction. However, their contribution to IL-32 biology during immune responses is hardly understood due to several isoforms with divergent inflammatory properties. Here, we identified IL-32 beta as the predominant isoform in various T cell subsets of healthy individuals and breast cancer patients with the highest levels detected in intratumoral regulatory T cells. We show that IL-32 beta is induced by IL-2 but IL-32 beta release requires T Cell Receptor rather than IL2R stimulation. Using inhibitors of protein secretion pathways and serial (ultra)centrifugation of T cell supernatants, we demonstrate that T cells actively secrete IL-32 beta unconventionally, as a free protein and, to a minor degree, through exosomes. Thus, our data identify activated T cells as major IL-32 beta secretors in health and cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; ACTIVATION; TRANSLOCATION; INFLAMMATION; SPECIFICITY; SULFATION; ISOFORMS; MONENSIN; RELEASE; PATHWAY; IL-32 isoform; IL-32 beta; IL-32 secretion mechanism; T cells; exosomes; unconventional secretion pathway; IL-2; cancer |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jan 2026 08:24 |
| Last Modified: | 20 Jan 2026 08:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64021 |
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