Karrer, Sigrid and Unger, Petra and Gruber, Michael and Gebhardt, Lisa and Schober, Robert and Berneburg, Mark and Bosserhoff, Anja Katrin and Arndt, Stephanie (2024) In Vitro Safety Study on the Use of Cold Atmospheric Plasma in the Upper Respiratory Tract. CELLS, 13 (17): 1411. ISSN , 2073-4409
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Cold atmospheric plasma (CAP) devices generate reactive oxygen and nitrogen species, have antimicrobial and antiviral properties, but also affect the molecular and cellular mechanisms of eukaryotic cells. The aim of this study is to investigate CAP treatment in the upper respiratory tract (URT) to reduce the incidence of ventilator-associated bacterial pneumonia (especially superinfections with multi-resistant pathogens) or viral infections (e.g., COVID-19). For this purpose, the surface-microdischarge-based plasma intensive care (PIC) device was developed by terraplasma medical GmbH. This study analyzes the safety aspects using in vitro assays and molecular characterization of human oral keratinocytes (hOK), human bronchial-tracheal epithelial cells (hBTE), and human lung fibroblasts (hLF). A 5 min CAP treatment with the PIC device at the "throat" and "subglottis" positions in the URT model did not show any significant differences from the untreated control (ctrl.) and the corresponding pressurized air (PA) treatment in terms of cell morphology, viability, apoptosis, DNA damage, and migration. However, pro-inflammatory cytokines (MCP-1, IL-6, and TNF alpha) were induced in hBTE and hOK cells and profibrotic molecules (collagen-I, FKBP10, and alpha SMA) in hLF at the mRNA level. The use of CAP in the oropharynx may make an important contribution to the recovery of intensive care patients. The results indicate that a 5 min CAP treatment in the URT with the PIC device does not cause any cell damage. The extent to which immune cell activation is induced and whether it has long-term effects on the organism need to be carefully examined in follow-up studies in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL-CELLS; PATHOGENESIS; APOPTOSIS; FIBROSIS; cold atmospheric plasma (CAP); plasma intensive care (PIC); pressurized air (PA); upper respiratory tract (URT); human oral keratinocytes (hOK); human bronchial-tracheal epithelial cells (hBTE); human lung fibroblasts (hLF) |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jan 2026 07:46 |
| Last Modified: | 26 Jan 2026 07:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64159 |
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