Muehler, Denise and Morini, Silvia and Geissert, Janina and Engesser, Christina and Hiller, Karl-Anton and Widbiller, Matthias and Maisch, Tim and Buchalla, Wolfgang and Cieplik, Fabian (2024) Stress response in Escherichia coli following sublethal phenalene-1-one mediated antimicrobial photodynamic therapy: an RNA-Seq study. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 23 (8). pp. 1573-1586. ISSN 1474-905X, 1474-9092
Full text not available from this repository. (Request a copy)Abstract
Since the molecular mechanisms behind adaptation and the bacterial stress response toward antimicrobial photodynamic therapy (aPDT) are not entirely clear yet, the aim of the present study was to investigate the transcriptomic stress response in Escherichia coli after sublethal treatment with aPDT using RNA sequencing (RNA-Seq). Planktonic cultures of stationary phase E. coli were treated with aPDT using a sublethal dose of the photosensitizer SAPYR. After treatment, RNA was extracted, and RNA-Seq was performed on the Illumina NextSeq 500. Differentially expressed genes were analyzed and validated by qRT-PCR. Furthermore, expression of specific stress response proteins was investigated using Western blot analysis.The analysis of the differential gene expression following pathway enrichment analysis revealed a considerable number of genes and pathways significantly up- or down-regulated in E. coli after sublethal treatment with aPDT. Expression of 1018 genes was up-regulated and of 648 genes was down-regulated after sublethal treatment with aPDT as compared to irradiated controls. Analysis of differentially expressed genes and significantly de-regulated pathways showed regulation of genes involved in oxidative stress response and bacterial membrane damage. In conclusion, the results show a transcriptomic stress response in E. coli upon exposure to aPDT using SAPYR and give an insight into potential molecular mechanisms that may result in development of adaptation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POSITIVE CONTROL; ENVELOPE STRESS; INACTIVATION; PROTEIN; MECHANISM; BACTERIA; PATHWAY; TRANSDUCTION; ANTIOXIDANT; INHIBITION; Antibacterial; aPDT; RNA-Seq; Escherichia coli; Stress response; Transcriptomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Nov 2025 10:04 |
| Last Modified: | 05 Nov 2025 10:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64279 |
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