Azzam, Saphia and Tomasova, Lea and Danner, Carina and Skiba, Michael and Klein, Maren and Guttenberg, Zeno and Michaelis, Stefanie and Wegener, Joachim (2024) A high-precision wound healing assay based on photosensitized culture substrates. SCIENTIFIC REPORTS, 14 (1): 9103. ISSN 2045-2322
Full text not available from this repository. (Request a copy)Abstract
Quantitative assessment of cell migration in vitro is often required in fundamental and applied research from different biomedical areas including wound repair, tumor metastasis or developmental biology. A collection of assays has been established throughout the years like the most widely used scratch assay or the so-called barrier assay. It is the principle of these assays to introduce a lesion into an otherwise confluent monolayer in order to study the migration of cells from the periphery into this artificial wound and determine the migration rate from the time necessary for wound closure. A novel assay makes use of photosensitizers doped into a polystyrene matrix. A thin layer of this composite material is coated on the bottom of regular cell culture ware showing perfect biocompatibility. When adherent cells are grown on this coating, resonant excitation of the photosensitizer induces a very local generation of 1O2, which kills the cells residing at the site of illumination. Cells outside the site of illumination are not harmed. When excitation of the photosensitizer is conducted by microscopic illumination, high-precision wounding in any size and geometry is available even in microfluidic channels. Besides proof-of-concept experiments, this study gives further insight into the mechanism of photosensitizer-mediated cell wounding.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SINGLET MOLECULAR-OXYGEN; TOCOPHEROLS; GENERATION; MIGRATION; LIFETIME; Cell migration; Wound healing; Photosensitizer; Singlet oxygen; Reactive oxygen species; Phototoxicity; Metastasis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Jan 2026 06:50 |
| Last Modified: | 15 Jan 2026 06:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64322 |
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