Vayssieres, Marlene and Juettner, Michael and Haas, Karina and Ancelin, Aurelie and Marchfelder, Anita and Leulliot, Nicolas and Ferreira-Cerca, Sebastien and Blaud, Magali (2024) RNase W, a conserved ribonuclease family with a novel active site. NUCLEIC ACIDS RESEARCH, 52 (21). pp. 13386-13401. ISSN 0305-1048, 1362-4962
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Ribosome biogenesis is a complex process requiring multiple precursor ribosomal RNA (rRNA) cleavage steps. In archaea, the full set of ribonucleases (RNases) involved in rRNA processing remains to be discovered. A previous study suggested that FAU-1, a conserved protein containing an RNase G/E-like protein domain fused to a domain of unknown function (DUF402), acts as an RNase in archaea. However, the molecular basis of this activity remained so far elusive. Here, we report two X-ray crystallographic structures of RNase G/E-like-DUF402 hybrid proteins from Pyrococcus furiosus and Sulfolobus acidocaldarius, at 2.1 and 2.0 & Aring;, respectively. The structures highlight a structural homology with the 5 ' RNA recognition domain of Escherichia coli RNase E but no homology with other known catalytic nuclease domains. Surprisingly, we demonstrate that the C-terminal domain of this hybrid protein, annotated as a putative diphosphatase domain, harbors the RNase activity. Our functional analysis also supports a model by which the RNase G/E-like domain acts as a regulatory subunit of the RNase activity. Finally, in vivo experiments in Haloferax volcanii suggest that this RNase participates in the maturation of pre-16S rRNA. Together, our study defines a new RNase family, which we termed the RNase W family, as the first archaea-specific contributor to archaeal ribosome biogenesis. [GRAPHICS] .
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARCHAEON HALOFERAX-VOLCANII; RIBOSOMAL-RNA; STRUCTURE REFINEMENT; MATURATION; INSIGHTS; PROTEIN; 5'-END; SYSTEM; GENE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jan 2026 11:17 |
| Last Modified: | 26 Jan 2026 11:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64426 |
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