Motewasselin, Nima and Hiller, Karl-Anton and Cieplik, Fabian and Kopp, Louis and Pfitzner, Arno and Pielnhofer, Florian and Auer, David L. and Buchalla, Wolfgang and Scholz, Konstantin J. (2024) Cerium- and samarium-nitrate interaction and accumulation on human dentin. ARCHIVES OF ORAL BIOLOGY, 167: 106053. ISSN 0003-9969, 1879-1506
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Objective: To investigate the accumulation of cerium-nitrate and samarium-nitrate on dentin without or with smear-layer and to test their antibacterial activity. Design: 24 dentin-enamel slices were cut from 24 extracted molars. 12 slices underwent smear-layer creation (320 grit, 200 g, 5 s), the other 12 smear-layer removal (20 % EDTA, 300 s). Slices were halved to 48 semilunar-shaped specimens. One specimen per tooth was treated with either Ce(NO3)(3) (50 wt% aqueous solution; pH = 1.29; n = 6) or Sm(NO3)(3) (50 wt% aqueous solution; pH = 1.88; n = 6). The other specimen served as control (A. demin). After water rinsing, elemental composition (Ce, Sm, Ca, P, O, N, Na, Mg, C) was measured (EDX; EDAX Octane-Elect, APEX v2.5, low-vacuum) in dentin. Atomic percent (At%), Ca/P- and Ca/N-ratios were calculated and analyzed non-parametrically (alpha = 0.05, error rates method). Additionally, antibacterial activity (2 min exposure) of Ce(NO3)(3) and Sm(NO3)(3) against Streptococcus mutans, Actinomyces naeslundii, Schaalia odontolytica, and Enterococcus faecalis was determined (colony forming units) after anaerobic incubation at 37 degrees C for 24 h (control: 0.2 % CHX). Results: At% (median) of Ce and Sm were as follows: Ce(NO3)(3) 3.4 and 0.9 At%Ce with and without smear-layer, respectively; Sm(NO3)(3) 2.4 and 1.3 At%Sm with and without smear-layer, respectively. Ce(NO3)(3) and Sm(NO3)(3)-application significantly decreased Ca/P-ratios (1.22 - 1.45; p <= 0.02) compared to controls (1.47 - 1.63). With smear-layer, significantly higher Ca/N-ratios (5.1 - 29.3) could be detected across all groups (p <= 0.004) compared to specimens without smear-layer (0.37 - 0.48). Ce(NO3)(3) and Sm(NO3)(3) showed reduction rates of up to >= 5 log10 steps for S. mutans, A. naeslundii, and S. odontolytica. Conclusions: Cerium and samarium nitrate showed accumulation on dentin and certain antibacterial activity and could therefore be identified as potential compounds to treat and prevent dentin and root caries and dentin hypersensitivity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ROOT CARIES; SMEAR LAYER; FLUORIDE; NANOPARTICLES; ENDODONTICS; MICROSCOPY; CHLORIDE; DENSITY; CE; Dentin; Smear-layer; EDX; Lanthanide; Cerium; Samarium; Nitrates |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller Chemistry and Pharmacy > Institut für Anorganische Chemie > Chair Prof. Dr. Arno Pfitzner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Dec 2025 09:36 |
| Last Modified: | 16 Dec 2025 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64456 |
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