Skoczynski, Kathrin and Kraus, Andre and Daniel, Christoph and Buettner-Herold, Maike and Amann, Kerstin and Schiffer, Mario and Hermann, Kristina and Herrnberger-Eimer, Leonie and Tamm, Ernst R. and Buchholz, Bjoern (2024) The extracellular matrix protein fibronectin promotes metanephric kidney development. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 476 (6). pp. 963-974. ISSN 0031-6768, 1432-2013
Full text not available from this repository. (Request a copy)Abstract
Complex interactions of the branching ureteric bud (UB) and surrounding mesenchymal cells during metanephric kidney development determine the final number of nephrons. Impaired nephron endowment predisposes to arterial hypertension and chronic kidney disease. In the kidney, extracellular matrix (ECM) proteins are usually regarded as acellular scaffolds or as the common histological end-point of chronic kidney diseases. Since only little is known about their physiological role in kidney development, we aimed for analyzing the expression and role of fibronectin. In mouse, fibronectin was expressed during all stages of kidney development with significant changes over time. At embryonic day (E) 12.5 and E13.5, fibronectin lined the UB epithelium, which became less pronounced at E16.5 and then switched to a glomerular expression in the postnatal and adult kidneys. Similar results were obtained in human kidneys. Deletion of fibronectin at E13.5 in cultured metanephric mouse kidneys resulted in reduced kidney sizes and impaired glomerulogenesis following reduced cell proliferation and branching of the UB epithelium. Fibronectin colocalized with alpha 8 integrin and fibronectin loss caused a reduction in alpha 8 integrin expression, release of glial-derived neurotrophic factor and expression of Wnt11, both of which are promoters of UB branching. In conclusion, the ECM protein fibronectin acts as a regulator of kidney development and is a determinant of the final nephron number.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BRANCHING MORPHOGENESIS; RENAL AGENESIS; BLOOD-PRESSURE; URETERAL BUD; NEPHRONECTIN; TUBE; IDENTIFICATION; GLOMERULOPATHY; MUTATIONS; HYPOXIA; Fibronectin; Alpha 8 integrin; Kidney development; Branching; Nephron number |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie > Prof. Dr. Ernst Tamm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Dec 2025 06:30 |
| Last Modified: | 10 Dec 2025 06:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64583 |
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