Hidrobo, Maria Soledad and Hoering, Marcus and Brunner, Sarah and Liebisch, Gerhard and Schweizer, Sabine and Klingenspor, Martin and Schreiber, Renate and Zechner, Rudolf and Burkhardt, Ralph and Ecker, Josef (2025) Cold-induced phosphatidylethanolamine synthesis in liver and brown adipose tissue of mice. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1870 (1): 159562. ISSN 1388-1981, 1879-2618
Full text not available from this repository. (Request a copy)Abstract
Increasing energy expenditure in brown adipose (BAT) tissue by cold-induced lipolysis is discussed as a potential strategy to counteract imbalanced lipid homeostasis caused through unhealthy lifestyle and cardiometabolic disease. Yet, it is largely unclear how liberated fatty acids (FA) are metabolized. We investigated the liver and BAT lipidome of mice housed for 1 week at thermoneutrality, 23 degrees C and 4 degrees C using quantitative mass spectrometry-based lipidomics. Housing at temperatures below thermoneutrality triggered the generation of phosphatidylethanolamine (PE) in both tissues. Particularly, the concentrations of PE containing polyunsaturated fatty acids (PUFA) in their acyl chains like PE 18:0_20:4 were increased at cold. Investigation of the plasma's FA profile using gas chromatography coupled to mass spectrometry revealed a negative correlation of PUFA with unsaturated PE in liver and BAT indicating a flux of FA from the circulation into these tissues. Betaadrenergic stimulation elevated intracellular levels of PE 38:4 and PE 40:6 in beige wildtype adipocytes, but not in adipose triglyceride lipase (ATGL)-deficient cells. These results imply an induction of PE synthesis in liver, BAT and thermogenic adipocytes after activation of the beta-adrenergic signaling cascade.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; HIGH-THROUGHPUT QUANTIFICATION; HORMONE-SENSITIVE LIPASE; FATTY-ACID; OXIDATIVE-METABOLISM; CHOLESTERYL ESTER; LIPID EXTRACTION; BEIGE ADIPOCYTES; MOUSE; WHITE; Cold exposure; Lipidomics; Lipolysis; Liver lipidome; Phosphatidylethanolamine; PUFA |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Mar 2026 10:45 |
| Last Modified: | 17 Mar 2026 10:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/64621 |
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